CONNECTIVE TISSUES. 345 



recommends for siaining fresh tissue Violet B, § 330. Elastic 

 fibres and smooth muscle also stain, but of different tints. 



DuBUEUiL (C. R. A-1S. Anat., vi Sess., 1904, p. 62) uses a 

 mixture of 23 vols. 1 per cent, picric acid and 2 vols. 

 1 per cent, methyl blue — with a foregoing stain with 

 carmalum or safranin. 



For Ranvier's method of artificial CEdemata for the study 

 of areolar tissue, see his Traite, p. 329. 



682. Unna's Orcein Method. — [Encych mik. Techn., 1910, 

 p. 250). Sections are stained for ten minutes in Griibler's 

 polychrome methylen blue. They are then washed with 

 water, mopped up, and brought for fifteen minutes into a 

 neutral 1 per cent, solution of orcein in absolute alcohol, 

 rinsed in pure alcohol, cleared in bergamot oil, and mounted. 

 Collagenous ground-substance dark red, muscle bluish, 

 elastic fibres sometimes dark red. Material may be fixed in 

 almost any way except with nitric or picric acid, formol, or 

 liquids of Miiller and Hermann. 



683. Unna's Methylen- blue -f Saurefuchsin (Unna, in Encycl. 

 mik. Technik, 1910, p. 247). Stain for 2—5 minutes in poly- 

 chrome methylen blue solution (Griibler). Wash and stain 

 for 10—15 minutes in " (0"5 per cent.) Saurefuchsin -f- (33 per 

 cent.) tannin-mixture (Grubler)." Water, alcohol, essence, 

 balsam. Collagen, protoplasm, and muscle red, nuclei and 

 keratin blue. On Flemming material, elastin blue. Liquids 

 of Hermann and Erlicki, formol and copper fixatives 

 incompatible. 



684. Unna's Safranin + Wasserblau [ibid.). Ten minutes 

 in 1 per cent, safranin. Wash. Ten to 15 minutes in ("1 per 

 cent.) Wasserblau + (33 percent.) tannin mixture." Wash. 

 Stains in opposite colours to the last. Formol a.nd liquid of 

 Hermann contra-indicated for fixing. 



685. Flemming's Orange Method is paid to give a very sharp 

 differentiation of developiiir/ fihrih. 



686. Malloey {Zeit. ?r!ss. Mile, xviii, 1901, p. 175) stains sections of 

 sublimate or Zenlcer material for a few minutes in Salirefnclisin of O'l 

 pel' cent., mordants for a few minutes in 1 per cent, phospliomolylijic 



