346 CHAPI'KR XXIX. 



acid and stains foi- 2 to 20 minutes in anilin blue 0'5 gvms., Orange G. 2, 

 oxalic acid 2. and water 100. His jjliospliotungstic lia^matoxylin stains 

 connective tissue sharply, but does not differentiate it sufficiently from 

 elastic tissue and muscle. 



687. For the complicated procedure of Hornowski see ihid.. xxvi, 



1909, p. 138. 



688. For Delamare's mixture of orcein, lifematoxylin, Siiurofuclisin 

 and picric acid see Vcih. Anat. Gen., xix, lOd.''., p. 227. 



689. Masson (C. B. Sor. Bull, Ixx, 1911. p. .573), stains first in 

 liaimalum, then in eosin, an<l tlien for a few minutes in 1 per cent, solu- 

 tion of saffron in tap water (made by boiling). Connective tissue, bone, 

 and cartilage, yellow. 



690. Benecke's stain for fibrils {Verh. Anat. Ges., vn, 189.3, 

 p. 10.5) is essentially that of Kijomayeb, § 656. 



691. Bielschowsky's Silver Me'I'HOD {jwsf, nndf^r 'Neuro- 

 fibrils') has been used for connective-tissue fibrils. Snicssaeew 

 (Av(d. Anz., xxxvi, 1910, p. 401) employs it as follows : 

 Tissue is hardened in neutral formol and sectioned with a 

 freezing microtome. The sections are pnfcfor at least 4 days 

 into iron alum of 2-5 to 10 per cent., changed daily. They 

 are then silvered for 36 to 48 hours in nitrate of silver of 10 

 per cent., then treated with the oxide bath and reduced in 

 formol of 20 per cent. Collagen fibres grey, but fine con- 

 nective networks black, nerve fibres unstained or only weakly 

 stained. 



See also Maeesch, Zeit. idns. Mik., xxiii, 1906, p. 356; 

 Studnicka, ibid., p. 416; Zimmermann, ibid., xxv, 1908, 

 p. 10; hsY I, Monit. zool. Ital., 1908, p. 290; Heinkich, 

 Arc]i. Mik. Anat., Ixxiv, 1909, p. 786 (dentine) : Insabato, 

 Arch. Ital. Anat. Emb., viii, 1909, p. 375 (silvers Flemming 

 material) ; Atpianasiu and Deagoiu, 0. R. Acad. Sci., cli, 



1910, p. 551 (Ramon y Ca.jal's silver process, with alcohol 

 fixation). 



Elastic Tissue. 



692. Elastic Tissue, Generalities.^ — Elastic fibres have a great 

 affinity for osmium, staining with much more rapidity than 

 most other tissue elements. They are not changed by 



