CONNECTIVE TISSTJRS. 347 



caustic soda or potash. They are normally acidophilous, but 

 are easily rendered artificially basophilous by means of 

 chromic acid or other mordants, and then stain with great 

 energy with basic dyes. Hence a group of stains of which 

 those of Lustgarten and Martinotti are types. They have a 

 natural affinity for orcein, whence stains of the Taenzer- 

 Unna type. 



For a review of the older methods of Balzee, Unna, 

 LusTGAui'EN, and TTbrxhkimee, sec the papor bj' G. Maetinotti, 

 in Zeit. iriss. Mil\, iv, 1887, p. 31 ; also I'mrycl. viil\ Teclinil\, 

 art. "Elastin." 



693. Victoria Blue (Lustgarticn). See § 289. 



694. Safranin (G-. IMartinotti, Inc. ciL, § 692). — Fix in a 

 chromic liquid, wash, stain for forty-eight hours in strong 

 (5 per cent. Pfitzner's) solution of safranin, wash, dehydi-ate, 

 clear, and mount in balsam. Elastic fibres black. 



The staining will be performed quicker if it lie done at the temperature 

 of an incubating stove (Geiesbach, -/hid., iv, 1887, p. 442). See also 

 Fbeeia {ihifl, V, 1888, p. 342). 



See also Mibelli, Mon. Zool. Italimio, 1, p. 17, or Zeit. whit. Mil;., vii, 

 1890, p. 22r) (the report in Journ. Roy. M'ic. Soc, 1890, p. 808, is 

 vitiated by a misprint). Other basic dyes have been recommended. 



695. Kresofachsin (Rothig, see § 289). 



696. Orcein. — This method is due to Taenzee, and as 

 modified by Unna is known as the Taenzee-Unna method, 

 see third edition, or Monatssch. 'prakt. Dermatol., xii, 1891, 

 p. 394. 



Unna's Modified Orcein Method [Monatssch. 'praht. Dermatol., 

 xix, 1894, p. 397; Zeit. wis.s. Milt., xii, 1895, p. 240).— 

 Griibler's orcein 1 part, hydrochloric acid 1 part, absolute 

 alcohol 100 parts. Stain sections for thirty to sixty 

 minutes, or for ten to fifteen at 30° C, rinse in alcohol, clear, 

 and mount. Elastin dark brown, collagen light brown. 



See alsoMerh. Siiz. Alcad. Wiss. PFMB.,cviii,1899,p. 335 ; Peantee,i6i(£., 

 xix, 1903, p. 361 (he takes 2 per cent, of nitric acid instead of the hydro- 

 chloric, and stains six to twenty-foni- hovirs) ; Wolff, ibid., p. 488 ; the 

 article "Elastin" in Encycl. mile. Technilc; and E. and T. Savini, Zeit. 

 wiss. Mih., xxvi, 1909, p. 34. 



