348 CHAPTEB XXIX. 



697. Weigert's Resorcin-Fuchsin Method {Gentralb. allg. 

 Path., ix, 1898, p. 290). — 1 per cent, of basic fuchsin and 2 

 per cent, of resorcin (or of carbolic acid) are dissolved in 

 water. 200 c.c. of the solution are raised to boiling-point 

 in a capsule, and 25 c c. of Liquor ferri sesquichlorati P. G. 

 are added, and the whole is boiled, with stirring, for two to 

 five minutes more. A precipitate is formed. After cooling 

 the liquid is filtered, and the precipitate which remains on 

 the filter is brought back into the capsule, and there boiled 

 with 200 c.c. of 94 per cent, alcohol. Allow to cool, filter, 

 make up the filtrate to 200 c.c. with alcohol, and add 4 c.c. 

 of hydrochloric acid. 



Woi.FRUM [Zeit. inss. Mik., xxv, 1908, p. 219) adds 10 to 

 1-5 per cent, of acetone to the mixture. 



Stain sections (of material fixed in any way) for twenty 

 minutes to an hour, wash with alcohol, clear with xjdol (not 

 with an essence). Elastic fibres dark blue on alight ground. 



MiNEBVJNi {Zeit. wiss. Mik., xviii, 1901, p. 161) gives a variant with 

 safranin instead of fuchsin. 



See also Prantee, ibid., xix, 1903, p. 361 ; B. Pischee, Virchow's 

 Arch., clxx, 1902, p. 285, or Zeit. wiss. Mik., xx, 1903, p. 40 (chemistry 

 of the dyes obtained by these processes, which he caUs "Pnchselin," 

 " Safranelin.'' etc.); Haet, Centralb. allg. Path., xix, 1908, p. 1; and 

 CiLiMBAEis, Arch. mik. Anat., Ixxv, 1910, p. 708. 



698. HsBmatoxylin Methods. — Hareis {Zeit. wiss. JWife., xviii, 1902. 

 p. 290) makes an " Elasthaematein '' as follows : Hsematoxyliu 0'2 grms., 

 aluminium chloride O'l grms., alcohol of 50 per cent. 100 c.c, boil and 

 add mercuric oxide 0'6 grma., filter and add 1 drop of HCl. Keep for 

 some weeks. Stain for five or ten minutes, put into alcohol with 1 per 

 cent, of nitric acid for one minute, then pure alcohol. 



See also Dh Witt, Anat. Bee, i, 1897, p. 74 ; Dtjeeck, Arch. Path. 

 Anat., clxxxix, 1907, p. 62; Yeehoepe, Journ. Amer. Med. Assoc, 1908, 

 No. 11. 



Malloey's phosphotungstic haematoxylin is good, but not specific. 



699. Other Methods for Elastic Tissue : 



For the elastic tissue of the skin see Passaege and Krosing, Derm. 

 Stud., xviii, 1894. 



See also for staining and dissociation Agababow, Arch. mik. Anat 1 

 ]897, p. 566 etseq. 



For 0. Martinotti's silver impregnation see Zeit. n-tss. Mik., v. 1888, 

 p. 521, or Arch. Ital. Biol, xi, 1889, p. 257. 



Schumacher {Arch. nnk. Anat.. Iv, 1899, p. 151) has had good results 

 (for the spleen) with picvn-nigrosin. § 681. 



See also § 733. 



