350 CHAPTER, XXIX. 



Arch., clxiv^ 190], p. 111). Ten minutes in the warm, 20° to 

 40 C.J in Griibler's carbol-pyronin-methyl-green mixture. 

 Cool rapidly, by plunging the recipient containing the tissues 

 into cold water. Remove the tissues with a platinum wire 

 and rinse. Absolute alcoholj bergamot oil, balsam. 



Stbopeni (Zeit. wiss. Mile, xxix, 1913, p. 302) takes iicridin red instead 

 of the pyroiiin. This will work after various fixatives. 



J3. — For small Plasma Cells. 



(4) As No. 2, supra, but only half a minute in the glycerin- 

 ether. 



(5) After removal of the celloidin from the sections with 

 alcohol and ether, five minutes in polychrome methylen blue, 

 wash, di'y with blotting-paper, dehydrate (about a minute) 

 in a mixture of 2 parts alcohol to 'd of xylol, then one 

 minute in xylol ; then 5 to 10 minutes in alum-anilin (pre- 

 pared by allowing anilin oil to stand over a layer of powdered 

 alum a couple of fingers deep) ; xylol, balsam. 



(6) As No. 3, swpira, after a foregoing stain of two minutes 

 in polychrome methylen blue. 



See also Ehklich in Virchow's Arch., clxxv, 1904, p. 198. 



703. Bhrlich's Original Method for Mastzellen {Arch. vdk. 

 Aiiat., xii, 1876, p. 263). — ^Stain, for at least twelve hours 

 in — ■ 



Absolute alcohol . . . 50 c c. 



Water . . . .100 c.c. 



Acid. acet. glacial . . 12-^- c.c. 



— to which has been added enough dahlia to give an almost 

 saturated solution. Wash out with alcohol, and mount in 

 resinified turpentine. 



See also Schibfferdecker and Kossbl's Gciveheh'hre, p. 329. 



704. Mastzellen, Unna's Latest Methods (Encycl. mik. 'J'echn., 

 1910, ii, p. 72). — (1) Stain three hours to overnight in poly- 

 chrome methylen blue with a knife-pointful of alum to a 

 watch-glass of the stain, rinse ; alcohol, oil, balsam. (2) 

 Stain in polychrome methylen blue quarter of an hour, rinse, 

 then ten minutes in glycerin-ether, § 702, wash thoroughly, 

 alcohol, oil, balsam. 



