CONNECTIVE TISSUES. 363 



solution, and stains in a couple of minutes. Wash out with 

 alcohol of 7U per cent. 



With either solution the staining- must be done in a 

 covered vessel or the stain will pi-ecipitate. 



Similarly Bell, Amer. Juurn. Anat., ix, 1909, p. 401, and 

 Anat. Rec, iv, 1910, p. 199. 



HuBXUEiMinit also {Geidralb. allij. Path., xiv, 1903, p. 841 ; 

 Zeit. wiss. Mik., xxi, 1904, p. 57) recommends a saturated 

 solution of the dye in a mixture of equal parts of acetone 

 and 70 per cent, alcohol. 



He also {De%dsche med. Wochenschr., xxvii, 1901, p. 607; 

 Zeit. triss. Mik., xix, 1902, p. 67) has had very fine results 

 by staining for twenty minutes in a saturated solution of 

 Indophenol in 70 per cent, alcohol. 



MoLLisoN {Zeit. wiss. Zool., Ixxvii, 1904, p. 529) has had 

 good results by staining gelatin sections for a few minutes 

 in strong extract of Alkanna in 96 per cent, alcohol, and 

 mounting in glycerin or syrup. 



LoKHAiN Smith [Juurn. Path. Bad., xii, 1907, p. 1) finds 

 that Nile blue stains fatty acids blue and neutral fats reddish. 



Similarly Eisenbkug {Virchow's Arch., cxcix, 1910, p. 502) 

 who recommends aqueous solution of Nilblau BB. 



Benda [ibid., clxi, 1900, p. 194) finds that free fatty acids 

 can be detected by Weigert's neuroglia mordant. See also 

 Bernee, ihid., clxxxvii, 1907, p. 360, and Fischleb, Zeit. wist>-. 

 Mik., xxii, 1905, p. 263. 



Oeajima [ibid., xxix, 1912, p. 67) extracts red capsicum 

 berries for some days with alcohol, and evaporates down to 

 one fifth. This stains only fatty bodies : amongst them, 

 myelin. 



See also Kingsbury, Anut. Rrc, v, 1911, p. 313. 



Buiic.* 



710. Bone, Non-decalcified. — Ranviidu [Traite, p. 297) has 

 the following : 



Bones should be plunged into water, without being allowed 

 to dry, as soon as the surrounding soft parts have been 



* For a detailed review of the whole subject, see the paper of 

 Schaffbe in Zeit. wiss. Mile, x, 1893, p. 167, or the article " Knoclien 

 und Ziihrie " in Encycl. iiiih. Tecliuile. 



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