358 CHAPTER XXIX. 



Ftjsari {Arch. Itiil. Biol., xxv, 1896, p. 200) makes sections of fresh 

 cartilage, jjuts tliem for twenty-four hours into 1 per cent, nitrate of 

 silver, washes, dehydrates, and exposes to the light in balsam. 



See also DisSB, Anai. Anz., xxxv, 1909, p. 31s, a stain for dentine, 

 (hsemalum followed by a mixture of Saiirerubin and Orange G) ; and 

 Rettbeee and Lblijsvbe, C. B. Soc. Biol., Ixx, 1911, p. 630. 



716. Cartilaginous Skeletons of emliryos (Van Wijhe, Proc. K. 

 Akad. Wetensch. Amstefdam, 1902, p. 17) may be studied by staining 

 embryos for a week in a solution of 0'2.5 gi-m. methylen blue in 100 c.c. of 

 70 per cent, alcohol with 1 per cent, of hydrochloric acid. Wash out 

 in alcohol with 1 per cent, of hydrochloric acid until no more colour 

 comes away (about a week) and mount in balsam. The cartilage 

 remains blue, all the other tissues being colourless. 



Similarly, Lundvall (Anat. Anz., xxv, 1904, p. 219, and xl, 1912, p. 

 639) using toluidin blue. 



Similarly also Bakay (Verh. Anat.Ges., 1902, p. 248), with Bismarck 

 brown (the embryos having been previously treated with nilric acid of 

 3 per cent.). 



