BLOOD AND GLANDS. 361 



minutes runs in osmic acid of 1 per cent.^ and after five 

 minutes more removes the cover. 



Dekhuysen {Anat. Anz., xix, 1901, p. 536) recommends a 

 mixture of either 3 or 9 vols, of 2 per cent, osmic acid with 

 1 of 6 per cent, acetic acid, containing ^ per cent, of methylen 

 blue, which he calls " Osmacet.'' 



The mercurial liquids of Pacini (§ 414) used to be con- 

 sidered good. Hayem {" Du Sang," etc., Paris, 1889; see 

 also Zeit. wis-". Mile, vi, 1889, p. 335) has the following 

 formula : sublimate 0"5, salt 1, sulphate of soda 5, and water 

 200. This should be mixed with blood in the proportion of 

 about 1 : 100. Eosin may be added to it. Lowri's formula 

 {ISitzb. k. Akad. Wiss. Wien, xcv, 1887, p. 144) consists of 

 5 c.c. cold saturated sublimate solution, 5 grms. sulphate of 

 soda, 2 grms. salt, and 300 c.c. water. Mosso finds that both 

 of these are too weak in sublimate. 



DuBOSCQ {Arch. Zool. Exper., vi, 1899, p. 481) uses (for 

 blood of Ghilopoda) a solution of acetic acid, copper acetate, 

 copper chloride, osmic acid, thionin, 1 grm. each, water 400, 

 which, mixed with tlie blood, fixes and stains in about two 

 miniites. 



Formol has lately been used. Maecano {Arch, de lied. 

 Exfer., xi, 1899, p. 434) mixes fresh blood with a mixture of 

 100 parts of sodium sulphate of sp. gr. r020 and 1 of formol ; 

 or with water 85 to 100 parts, sodium chloride 1, and 

 formol 1. 



KizEB {Journ. Buy. Mic. 8oc., 1900, p. 128) simply mixes 

 1 drop of blood with 3 o£ 2 per cent, formalin, and allows to 

 stand for an hour. 



ScHRiDDE {Hxmat. Techv. Jena, 1910, p. 17) lets blood drop 

 into a mixture of I part of formol, 9 of liquid of Muller, and 

 10 of water, fi\es therein for 2 to 4 hours at 40° C, filters, 

 washes and brings through alcohol and chloroform into 

 paraffin for sectioning. 



719. Fixing and Preserving in films. — Muie {Journ. of Anat. 

 and Phys., xxvi, 1892) makes cover-glass films and drops 

 them into saturated sublimate solution, and after half an hour 

 washes, dehydrates, and passes through xylol into balsam. 



GuLLAND {Brit. Med. Joum., March' 13th, 1897; Scotthh 

 Med. Journ., April, 1899) makes cover-glass films, and after 



