BLOOD AND GLANDS. 365 



establishment of a process worked out by Giemsa as perhaps 

 the most trustworthy and efficient of "Romanowsky" stains. 

 This is as follows : 



Giemsa's Azur-eosin process. You start with a mixture of 

 eosin with metliylenaztor (instead of methylen blue). This 

 mixture is very troublesome to prepare, and is best obtained 

 ready made from Griibler & Hollborn (their "Giemsa'sche 

 Loesung fiir Romanowsky-faerbung "*). Air -dried films 

 {Deutsch. med. WocJiensclir., 1907, No. 17) are fixed in 

 alcohol or in methyl-alcohol (two to three minutes), and dried 

 with blotting paper. They are treated for ten to fifteen 

 minutes with a dilution of 1 drop of tlie stock mixture to 

 1 CO. of water, washed iindei' a tap, dried with blotting- 

 paper, and again dried in the air and mounted in balsam, or 

 (preferably) preserved unmounted. AH reagents, especially 

 the balsam, must be &ivici}y free from arid. 



Wet films (ibid., 1909, p. 1751) are treated as follows: 

 Fix them for twelve to tvveuty-four hours in a mixtui-e of 2 

 parts saturated aqueous solution of sublimate with 1 of 

 absolute alcohol. Wash, and treat for five to ten minutes 

 with a mixture of 2 parts of iodide of potassium, 100 of 

 water, and 3 of Lugol's solution. Wash, and treat for ten 

 minutes with 0'5 per cent, solution of sodium thiosulphate. 

 Wash, and stain as above (changing the stain for fresh after 

 half an hour), for one to twelve hours. Then pass through 

 mixtures of acetone with first 5, then 30, then 50 parts per 

 cent, of xylol into pure xylol, and mount in cedar oil. This 

 process is applicable to sections. 



Or [ibid., 1910, p. 2476) a slide is placed in a Petri dish 

 and covered with a mixture of equal parts of metliy I- alcohol 

 and stock mixture. After half a minute this is poured off 

 and enough distilled water poured in to cover the slide, and 

 the whole is rocked to mix the two. After three to five 

 minutes, wash in running water, dry, and mount in cedar 

 oil. 



By any of these processes nuclei (i-ed) are demonstrated 

 not only in hasmatozoa, but, in many bacteria, spirocha3ta5, 

 coccidia, sarcosporidia, etc. 



* To make this up from Griibler's powdei's, dissolve 3 grms. of 

 Azur Il-eosin and 8 decigrammes of Azur II, in 12-5 gvms. of glycerin 

 and 375 of methyl-alcohol. 



