366 CHAPTER XXX. 



See also, for paraffin sections, Schubeeg, in Deutsch. med. 

 Wochenschr., xxxv, 1909, No. 48, or Zeit. loiss. Mik., xxvii, 

 1910, p. 161, who passes through acetone and xylol into 

 balsam. 



The older Romanowsky stains published by the authors 

 mentioned above, as also Laveran's " Bleu Borrel " seem to 

 be superseded by Giemsa's. 



Leishman's Romanowsky Stain [Brit. med. Journ., March 

 16th and September 21st, 1901) is as follows : To a 1 per cent, 

 solution of Griibler's medicinal methylen blue in water add 

 0'5 per cent, of sodium carbonate, heat to 65^ 0. for twelve 

 hours and let stand for ten days. Then add an equal 

 volume of O'l per cent, solution of Griibler's Eosin extra B, 

 let stand for six to twelve hours, collect the resulting pre- 

 cipitate on a filter, wash it until the wash water comes off 

 colourless, di'y and powder. For staining, dissolve 0"]5 grm. 

 in 100 c.c. of pure methyl alcohol. Stain cover-glass films 

 (air-dried) for five to ten minutes ; flood the film with water 

 for one minute, and examine, or dry (without heat) and 

 mount in xylol balsam. Nuclei in shades of red, cytoplasm 

 bluish, parasites blue with ruby red chromatin. 



Raadt (MiHic/i. med. Wochenschr., 1911, No. 27 ; Zeit. 

 wlss. Mik., 1912, p. 236) obtains a Romanowsky stain of 

 blood and parasites with Jbnneb's solution. Films fixed 

 with alcohol and ether are fii'st stained for five to ten 

 minutes in solution of one part methylenblau med. puriss. 

 Hoechst, 0'5 part of lithium carbonate and 100 of water, 

 kept for at least three weeks and diluted with 10 vols, of 

 water. Rinse with water, dry with blotting paper, flood 

 with Jenner's solution diluted with 2 or 3 vols, of water, 

 and stain for five to ten minutes. Wash, dry with blotting 

 paper, and mount. 



721. Pappbnheim (Anat. Anz., xlii, ]912, p. 525) recommends the 

 following for sections of hxinopoietic tissues, and also of kidney, liver, 

 hypophysis, suprarenals, lung, intestinal epithelium and centi-al nervous 

 system. Fix in Orth's Formol-Miiller, stain sections for twenty minutes 

 in a stove in " aqueous diluted alcoholic " solution of jUay-Gettnwald 

 or Jbnnbk diluted with 8 vols, of water; after-stain for forty minutes 

 in the stove in " aqueous Gibmsa solution (15 c.c. of water with 10 

 drops of glacial acetic acid) " ; differentiate in 100 c.c. of water with 

 5 to 6 drops of acetic acid ; wash, dry between blotting-paper ; dehydrate 

 in mixture of equal parts of acetone and\ibsolute alcohol, and mount in 



