BLOOD AND GLANDS. 367 



neutral balsam. The result is not a Romanowsky stain, but a pale 

 methylen-blue-eosin stain. 



See also Wright, Pub. Massachusetts Gen, Hasp., iii, 1910, p. 1, or 

 Journ. B. Micr. Soc, 1910, p. 783. 



For the special technique of eosinophilous cells see Mabtinotti in 

 Zeit. wiss. Mikr., xxvi, 1909, p. 4 (alphabetical bibliogi-aphy of eight 

 pages.) 



722. Demonstration of Blood-plates of Bizzozero (Kemp, Studies 

 from the Biol. Lab. Johns Hopkins Univ., May, 1886, iii, No. 6 ; 

 Nature, 1886, p. 132). — A somewhat large drop of blood is 

 placed on a slide, and quickly washed with a small stream of 

 normal salt solution. The blood-plates are not washed away, 

 because they have the property of adhering to glass. They 

 may be stained with solution of 0'02 per cent, of methyl 

 violet or 1 : 3000 of gentian violet, in salt solution. To 

 make permanent preparations of them, they should first be 

 fixed, by putting a drop of osmic acid solution on the finger 

 before pricking it. 



They may also be stained in films, especially by the 

 Romanowsky method. According to Pappenheim (" Farb- 

 chemie," p. 107) Wasserblau is almost specific for them. 



Weight {Journ. Morph., xxi, 1910, p. 274) studies them in 

 tissues, after fixation with formol or sublimate (not Zenker) 

 by staining with a modified Griemsa stain, and bringing 

 through acetone and oil of turpentine into turpentine 

 colophonium. Details loc. clt. or Journ. Roy. mic. Soc, 

 1910, p. 783. 



See also Dekhuysen, Anat. Anz., xix, 1901. p. 533 ; 

 KoPSCH, Intern. Monatschr. Anat. Phys , xxi, 1904, p. 344, 

 and xxiii, 1906, p. 359 ; Deetjen, Zeit. phys. Chem., Ixiii, 

 1909, p. 1. 



723. Wbigeet's Fibrin Stain {Fortschr. d. Med., v, 1887, 

 No. 8, p. 228). — Sections (alcohol material) are stained in a 

 saturated solution of gentian or methyl violet in anilin water 

 (§ 286). They are brought on to a slide and mopped up 

 with blotting-paper, and a little Lugol's solution is poured 

 on to them. After this has been allowed to act for a sufficient 

 time they are mopped up with blotting-paper, and a drop of 

 anilin is poured on to them. The anilin soon becomes dark, 

 and is then changed for fresh once or twice. The auiiin is 



