368 CHAPTER XXX. 



then thoroughly removed by means of xylol, and a drop of 

 balsam and a cover are added. This stain may be applied 

 to celloidin sections without previous removal of the celloidin. 



See also the modifications of tins method by Kromayer (§ 6.56) ; 

 Benbckb (§ 690) ; Unna (Monatssch. pralct. Dennat, xx, 1895, p. 140) ; 

 Wolff {Zeii. wiss. Mile, xv, 1899, p. 310) ; and one of another sort by 

 KoCKEL, Centralb. allg. Path., x, 1899). 



Glands. 



724. Mucin. — IIoyee {Arch. mih. Anal., xxxvi, 1890, p. 310) 

 finds that the mucin of mucus cells and goblet cells stains 

 with ia.sic tar colours and with alum haamatoxylin, but not 

 with acid tar colours. He obtained his best results ))y means 

 of thionin, and good ones with toluidin blue, both of these 

 giving a metachromatic stain — tissues blue, mucin reddish — 

 and also with methylen blue (which is particularly useful 

 from its power of bringing out the merest traces of mucin), 

 safranin, etc. 



Tissues should be fixed for two to eight hours m 5 per 

 cent, sublimate solution, and paraffin sections stained for 

 five to fifteen minutes in a very dilute aqueous solution of the 

 dye (two drops of saturated solution to 5 c.c. of water). 



Hyaline cartilage, the jelly of Wharton, and the Mastzellen 

 of Bhrlich give the same reactions with basic dyes as mucin 

 does. 



See also SassDOEF, Deutsche. Zeit. Thiermed., xiv, pp. 345, 

 349 (Zeit. viss. Mile, vi, 1889, p. 205) ; Bizzozeeo, AH I. U. 

 Accad. di 8ci. di Torino, 1889 to 1892 (reports in Zeit. wiss. 

 Mih., vii, 1890, p. 61 ; and ix, 1892, p. 219) ; also Unna, ihid., 

 xiii, 1896, p. 42. 



The safranin reaction is not obtained with all brands of 

 the dye ; that of Bindschedler and Busch, in Bale, gives it, 

 whilst safranin of Griibler does not. Unna employs chiefly 

 polychrome methylen blue. 



As regards the thionin stain, see Hari, Arch. Mih. Anat., 

 Iviii, 1901, p. 678. 



Bkuno [Bull. Soc. Nat. Napoli, 1905, p. 220) fixes and 

 stains the skin of the frog in a mixture of 100 c.c. of formol 

 of 1"25 per cent, with 8 c.c. of ] per cent, solution of thionin. 

 Mucus glands red. 



