370 CHAl'TER XXX. 



Geand-Mouksel and Tkibondkau [C. B. Soc Biul., hii, 

 1901, p. 187) recommend for pancreas Nicof.LE'a " thionine 

 plieniquee/' wliich stains tlie insula3 of Langerhans liardly at 

 all J tlie rest strongly. 



730. Gastric Glands.— Kolstur (Zcit. wis^. Mile, xii, 1895 

 p. 314) differentiates the two kinds of cells in stoniacli 

 glands by over-staining with luBmatoxylin, washing out with 

 alcohol containing 1 per cent, of HCl, blueing with alcohol 

 containing 1 per cent, of ammonia, and, after washing, 

 staining for one to live minutes in a weak solution of 

 Saurefuclisin. Peptic cells blue, parietal cells red. Osmic 

 material cannot be employed. 



Cade [Arch. Anat. Mkr., iv, 1901, p. 4) stains material 

 fixed with Bouin's picroformol in Victoria blue of 1 per cent, 



R. and L. Monti {Rich. Lab. Anat. Roma, ix, 1902) demon- 

 strate ducts and canaliculi of delomorphous cells by Golgi's 

 bichromate and silver impregnation, especially with reju- 

 venated material (see Sacehdotti), leaving it for 5 or C days 

 in half-saturated sulphate of copper, then for 24 hours in the 

 osmic-bichromate mixture. You can imbed in paraffin 

 (rapidly). 



731. Intestine. — Bjsnsley [Aiaei-. Journ. Anat., v, 1906 p. 

 323) stains sections of glands of Lieberkiihn in a mixture 

 of equal parts of satui-ated solutions of Orange G and 

 Saurerubin, and then with toluidin blue, and mounts -in 

 balsam . 



732. Liver. — Bkaus {Denlcschr. Med. Nat. Oei<. Jena, v, 

 1896; p. 307) demonstrates the bile capillaries by the rapid 

 method of Golgi, hardening in a mixture of one part forniol 

 with three parts liquid of IMuUer or J per cent, ohi^omic 

 acid. 



Eppingeb {Beitr. path. Anat., xxxi, 1902, p. 230) studies 

 them by means of a complicated modification of Weigeet's 

 neuroglia stain, and Ciechanowski {Anat. Anz., xxi, 1902, p. 

 426) by means of Weigekt's myelin stain (the 1885 method). 



Oppel {Anat. Ana., v, 1890, p. 144; vi, 1891, p. 168) puts 

 pieces of liver or spleen (alcohol material) for twenty-four 

 hours into a solution of neutral chromate of potash (4 to 



