BLOOD AND GLANDS. 371 



10 per cent.), then for twenty-four hours into a J per cent. 

 solution of silver nitrate, washes, dehydrates, and cuts 

 without imbedding. The lattice fibres are only stained near 

 the surface, so that tangential sections must be made. 



Similarly Berkley, ibid., 1893, p. 772, fixing in picric 

 acid, then in an osmium bichromate mixture, and then 

 silvering. 



See also Ranvibe, Journ. de Microgr., ix, x, 1885-6 ; Igacuschi, in 

 Arch. path. Anai., xcvii, p. 142, or Zuit. wiss. Mile, 1885, p. 243 (gold 

 process for study of fibrous networks) ; Kupffbe, Sitzh. Oes.f. Morph., 

 etc., Miinchen, Juli, 1889, or Zeit. wiss. Mile., vi, 1889, p. 506 ; Kkatise 

 {Arch. mile. Anat., xlii, 1893, p. 57) ; and Timofejew, Anai. Anz„ xxxv 

 1909, p. 296 (sections of frozen tissue stained with methylen blue). 



733. Spleen. — For lattice fibres, see Opi^el, last §. 

 KuLTSCHiTZKY (Arch. mih. Anat., xlvi, 1895, p. 675) studies 



the musculature in sections (of material from liquid of 

 Miiller) stained for a day or more in a solution of lakmoid 

 in ether and mounted in balsam. 



For elastic fibres he puts sections for half an hour or a 

 day into a mixture of 800 parts 96 per cent, alcohol, 40 parts 

 1 per cent, solution of carbonate of potash, 2 parts Magdala 

 red, and 1 part methylen blue. 



For the blood-vessels he puts sections of Miiller material 

 for a few minutes into a solution of one or two parts of 

 Saurerubin in 400 parts of 3 per cent, acetic acid, washes 

 out in 2 per cent, acetic acid, and after-stains in a similar 

 solution of helianthin or Wasserblau until the red only 

 remains in the erythrocytes. 



See also Whiting {Trans. Roy. Soc, Edinburgh xxxviii, 1896, p. 311) ; 

 ScHUMAOHEB {Arch mile. Anat., Iv, 1899, p. 151) ; Weidbnbbich {ibid., 

 Iviii, 1901, p. 251). 



734. Lymphatic Glands. — For lattice-fibres especially, see 

 RoEBSLE & YosHiDA, Beitr. 'path. Anat., xlv, 1909, p. 110, or 

 Zelt. wiss. mik., xxvi, 1909, p. 295. Sections stained with 

 haematoxylin and eosin, or Weigert's iron hasmatoxylin, or 

 Bielschowsky's neurofibril stain as applied by Maeesch, loc. 

 cit., § 691. The sections should not remain for more than 

 15 to 30 minutes in the oxide bath. 



