NEEVODS SYSTlOM— GfiNEKAL METHODS. 385 



of water, and 100 of alooliol, washes with alcohol, clears with origanum 

 oil, and mounts. 



751. Alizarin. — Schkottek {Neurol. Centrulb., xxi, 1902^ 

 p. 338 ; Znt. wiss. iliJi., xis, 1903, p. 381) stains sections 

 for twenty-four hours in a 1 to "2 per cent, solution of sul- 

 plializarinate of soda, differentiates for |- to 1 minute in 

 tap-water, dehydrates, and mounts. This is a general stain, 

 but demonstrates Nissl bodies and other internal details. 



752. Nissl's methylen-blue is used as a general stain by some. 



ROTHiG (Folia Neurobiol, ii, 1909, p. 385 ; Zeit. wiss. Mile, xxvi, 1909, 

 p. 282) fixes and stains for about four weeks in saturated solution of 

 Mcthijlenazur I (Gi-iibler) in formol of 10 per cent., puts for ten to fifteen 

 miniites into aceton, then for twelve hours into chloroform, and imbeds 

 in paraffin. He also has a process with trichloracetate of lead and 

 methylenaziir. 



Bawitz [Zeit. wiss. Mile, xxvi, 1910, p. 341) has sonae complicated 

 methods with Indulin, Indamin blue, and Azosdureblau, which take 

 twenty-eight days ; and [ibid., xxviii, 1911, p. 1) others -with, fuclisin and 

 azofnchsin which take over thirty-six days. 



Kappbes {ibid., p. 417) describes a stain of chromic material with 

 extract of elderberries. 



SCARPATETTI {Neurol. Centralb., xvi, 1897, p. 211 ; Zeit. wiss. Mile, 

 xiv, 1897, p. 91) stains sections of formol material for five minutes in 

 1 per cent, heematoxylin, treats for five minutes with concentrated 

 solution of neutral acetate of copper, differentiates with Weigert's 

 borax-feri'i-oyanide, treats with concentrated solution of carbonate of 

 lithia, washes and mounts. Myelin is not stained. 



753. MALiOEy's Phospho-molybdic-acidHsematoxylin has been - 

 given, § 271. 



For the extremely complicated modification of Aueebach, see Neurol. 

 Centralb., xvi, 1897, p. 439, or Zeit. wiss. Mile, xiv, 1897, p. 402, and for 

 that of KoDis see § 271. 



754. Heematoxylin and Siurefuchsin. — Finotti {Virchow's Arch., 

 cxliii, 1896, p. 133 ; Zeit. wiss. Mik., xiii, 1896, p. 236) stains in hasma- 

 toxylin, counter-stains for three minutes with O'S to 1 per cent, solution 

 of Saurefuchsin, and differentiates in 75 per cent, alcohol containing a 

 very little caustic potash. 



Van Gibson's hematoxylin and picro-Saurefuchsin, § 398, gives 

 useful general views of nerve-cells, axis-cylinders, and neuroglia. 



755. Alt (-Miotic/!, med. Wochenschr., 1892, No. 4; Zeit. wiss. Mile, 

 ix, 1, 1892, p. 81) stains for a couple of hours in solution of Congo red 

 in absolute alcohol, and washes out with pure alcohol. For peripheral 

 axis-cylinders, and other elements. 



25 



