NEltVOUS SYSTEM — CYTOLOGICAL METHODS. 387 



10 parts of anilin oil with 90 pai-fcs of 96 per cent, alcohol, 

 and as soon as no more colour is given off from them are got 

 on to a slide, dried with filter-paper, cleared with oil of 

 cajeput, dried again with filter-paper, treated with a few 

 drops of benzin, and mounted. 



Van Gehuchten {in litt.) prefers to take 'paraffin sections, 

 mounted on slides by the water method, and stain them for 

 five or six hours in Nissl's mixture in a stove kept at 35° to 

 40° C. 



Rehm [Miiiich. med. Wochenschr., 1892, No. 13 ; Zeit. wiss. 

 Mlk., ix, 1893, p. 387) stains for half a minute to a minute 

 in a hot O'l per cent, of methylen blue, washes in 96 per cent, 

 alcohol till no more colour comes away, clears with origanum 

 oil, and mounts in balsam. 



GoTHAED {C. B. Soc. Biol, V, 1898, p. 530) stains cul- 

 loidin sections for twenty-four hours, without heat, in Unna's 

 polychromatic methylen blue and differentiates in a mixture 

 of 5 parts of creosote, 4 of oil of cajeput, 5 of xylol, and 16 

 of absolute alcohol. 



LuiTHLEN and Sorgo {Neurol. Centralb., xvii, 1898, p. 640) 

 differentiate in Unna's glycerin-ether mixture (§ 702), 

 remove this with absolute alcohol, and clear in origanum 

 oil. 



Lennhoi^f {ihith, 1910, p. 1) recommends polychrome 

 methylen blue for 2 minutes, followed by Griibler's " Karbol- 

 Methylgriin-Pyronin " for 20 minutes. 



LoKD {Journ. Ment. Sci., October, 1898) makes sections of 

 fresh tissue, frozen, treats them for a few seconds with a 

 mixture of equal parts of 6 per cent, formaldehyde and 

 saturated solution of picric acid, then rinses, and warms till 

 bubbles appear in 5 per cent, solution of methylen blue. 



Mentz von krogh {Centralb. Bakt.,\Ym, 1911, p. 95) stains 

 paraffin sections for 5 minutes in polychrome methylen blue, 

 treats for 1 to 15 with 2 per cent, chromic acid, differentiates 

 till blue with 5 per cent, tannin, and mounts in balsam. 

 Shows also axis cylinders. 



See also Goldscheidee & Flatatt, Normale und. path. Anai. der 

 Nerveiizellen, etc., Berlin, Kornfeld, 1898 {Zeit. wiss. Mile, xvi, 1899, 

 p. 102), and Nissl's remarks thereon, Deutsche Zeit. Nervenheilh., xiii, 

 1899, p. 3-18 {Zeit. wiss. Mih., xvi, 1899, p. 370) ; Cox, Intirii. Monatsschr. 

 Anat. Phya., xv, 1898, Heft 8 ; Myees, Anat. Record, 1908, p. 434 ; 



