388 OlIAPTEE XXXlt, 



Ilbbeg, Neurol. Ceidralb., 1896, No. 18; Savini, CentraLb. Bakt,, 1909, 

 p. 697. 



759. MethylenBlueandErythrosin. — Held {Anat. l'hys.,Anat. 

 Ahth., 1895^ 1896, p. 399) stains sections on slides, with tlie 

 aid of a gentle heat, for one or two minutes in a solution of 

 1 grm. of Griibler's erythrosin in 150 of water with two 

 drops of glacial acetic acid, washes out with water, and 

 stains in a mixture of equal parts of Nissl's methylen blue 

 and 5 per cent solution of acetone, warming strongly the 

 while, until all odour of acetone has disappeared. After 

 cooling he differentiates with O'l per cent, solution of alum 

 until the sections appear reddish, rinses in water, dehydrates 

 as rapidly as possible in absolute alcohol, and passes through 

 xylol into balsam. See further hereon Held, op. cit., 1897, 

 pp. 226 — 233, 273 — 305 (Supplementband), and Boccakdi, 

 Mon. Zool. Ital., x, 1899, p. 141 (stains in a mixture of 

 erythrosin O'l, toluidin blue, 0'2, and water 100 parts, and 

 differentiates in 0'5 per cent, alum solution). 



760. TMonJn. — Lenhossek (Fein. Bau. d. Nervensystevis, 

 Berlin, 1894, p. 149) stains sections of formol material for 

 five minutes in a concentrated aqueous solution of thionin, 

 rinses with water, and mounts as Nissl. The stain does not 

 keep well. 



Similarly Bamon y Cajal, Man. de Anat. Path. Gen., 1896 

 (see Zeit. wiss Mile, xv, 1899, p. 375), and Luxenbukg, 

 Neurol. Centralb., xviii, 1899, p. 629. 



761. Toluidin Blue. — Lenhossek (Neurol. Centralb., xvii, 

 1898, p. 577 ; Zeit. wiss. Mih., xv, 1899, p. 492). Sections 

 are stained on slides for a night in concentrated solution of 

 toluidin blue, rinsed in water, quickly differentiated with 

 alcohol, cleared with xylol or cai-bolic-acid xylol, and mounted 

 in balsam. 



Similarly Polumobdwikow {^Zeit. wiss. Mik., xvi, 1899, 

 p. 371, who stains in a very weak alkaline solution, 1 part of 

 1 per cent, solution to 119 of water and 1 of carbonate of soda. 



762. Neutral Red.— JuLiDSBUEGEE (Neurol. Centralb., xvi,, 

 1897, p. 259) stains sections of formol material, for half to 

 three quarters of a minute in ivarm 1 per cent, solution of 



