CHAPTER XXXIII. 



MYELIN STAINS. 



779, Iron Hsematoxylin. — I find the simplest way to make 

 a myelin stain is to make paraffin sections of formol material 

 and stain them with iron heematoxylin, exactly as for central 

 corpuscles (say twelve to fourteen hours in the mordant, six 

 in the hasmatoxylinj and about two minutes for the differen- 

 tiation). Sections best not over 15 /x. You may after-stain 

 the cells (which are only grey) with carmalum, but not for 

 more than half an hour, or the heematoxylin will be attacked. 

 The stain is not so testhetic as Weigert^'s, but quite as sharp. 

 Axis cylinders are not shown. 



Similarly Regaud, C. R. Acad. 8ci., clxviii, 1909, p. 861, 

 but adding a chrome mordantage either concurrently with the 

 formol fixation, or subsequently. 



xVlso Naseotte, C. R. Sue. Biol , Ixvii, 1909, p. 542, with 

 sections of formol material by thu freezing method; Hohsek, 

 Journ. Comp. Neurol, and Psych., x, 1901, p. 65, and 

 Bkookovek, ibid., xx, 1910, No. 2 ; Spielmeyee, Neurol. 

 Zentralb., xxix, 1910, p. 348, and his Technik. d. viikro. 

 JJntersuch. d. Nerven>''ystems, 1911, p. 87, with sections of 

 25 to S5;it by the freezing method; Loyez, G. R. Soc, Biol., 

 Ixix, 1910, p. 511, who differentiates first lightly, till the 

 grey begins to come out, in the iron alum, then washes, 

 and differentiates further in Weigert's borax ferricyanide ; 

 Gli.beet, Zeit. uiss. Mik., xxviii, 1911, p. 279, who mordants 

 with iron alum, stains with molyhdlc acid hsematoxylin, and 

 differentiates with the borax ferricyanide ; Stoelznee, ibid., 

 xxiii, 1906, p. 329, who mordants celloidin sections of formol 

 material for five minutes in Lig. Jerri sesquichlorati, stains 

 in hEematoxylin of 0'5 per cent., and differentiates in the 

 mordant or in borax ferricyanide; and KoDiS, Arch. mik. 

 Anat., lix, 1901, p. 211, who fixes for one or two days in 



