MYELIN STAINS. 413 



lionrs, after which the sections arc clipped in solution of 

 Muller, and differentiated by the method of Pal. 



Similarly Kaes {ibid., vm, 1891, p. 388 ; Nevrol. Gentralh., 



1891, No. 15). Myelin dark blue, cells yellow-brown. 



786. MiTEOPHANOW {Zeil. wise. Mile., xiii, 1896, p. 361) mordants 

 photoxylin sections for at least twenty-fom- hours at 40° C. in a mixture 

 of equal parts of saturated aqueous solution of acetate of copper and 90 

 per cent, alcohol, stains for ten minutes in Kultschitzky's haematoxylin, 

 and differentiates with Weigert's femcyanide. 



787. Behkley's Rapid Method {Neurol. Gentralh., xi, 9, 



1892, p. 270; Zeit. uns<. Mile, x, 1893, p. 370).— Slices of 

 tissue of not more than two and a half millimetres in thick- 

 ness are hardened for twenty-four to thirty hours in mixturr 

 of Flemming, at a temperature of 25^ C, then in absolute 

 alcohol, then imbedded in celloidin and cut. After washing 

 in water the sections are put overnight into a saturated 

 solution of acetate of copper (or simply warmed therein to 3.5° 

 to 40 C. for half an hour). They are then washed, and 

 stained for fifteen to twenty minutes in a lithium carbonate 

 hEematoxylin similar to Weigert's, warmed to 40° C, allowed 

 to cool, and differentiated for one to three minutes in Weigert's 

 ferricyanide liquid, which may be diluted if desired with one 

 third of water. 



788. Hill {Brain, 1896, p. 1 ; Phil Trans., 184, b, 1894, 

 p. 399) stains well-washed Miiller material in bulk in alum 

 carmine, cuts, mordants for twenty-four hours in half- 

 saturated acetate of copper, stains and differentiates as 

 Weigert, taking the differentiating fluid only half as strong. 



789. Benda's Rapid Method {Berlin, hlin. Woehensehr., No. 32, 

 1903). — Sections of formal material by the freezing process (alcohol 

 being avoided) are stained (without any mordanting) for twenty-four 

 hours in Boehmer's haematoxylin, differentiated with Weigert's ferri- 

 cyanide, and mounted in balsam. Only recommended for peripheral 

 nerves, or for preliminary examination of the central nervous system. 



Similarly, Nageotte, C. B. Soc. Biol., 1908, p. 408, staining with 

 hsemalum. 



Similarly the Encycl. mile. Tcchnih., 1910, ii, p. 239, viith. fresh material 

 cut by the freezing process, and the sections mounted in levulose (as 

 alcohol somewhat extracts the stain). 



