414 OHAPTKu xxxni. 



790. SxEisETisit {An-Ji. Mih. Anab., Ixii, 1903, p. 734) stains 

 smull neri-e-centre-'i in bulk (after mordanting in Weigert's 

 bichromate and fluoride mixture, § 781) with Weigert's 

 hsematoxylin (foar to six days), washes for a couple of days 

 in 70 per cent, alcohol, makes paraffin sedioiif, and differ- 

 entiates them by the method of Weigert or Pal. 



791. Besta [Zeit. wiss. Mih., xxiv, 1907, p. 185) mordants nei-ves for 

 one to three days in 100 c.c. of water with 2.5 of foi-mol and 4 grms. of 

 Merck's ammonio- chloride of tin, cuts in paraffin, stains in Malloey's 

 hsematoxylin and diiferentiates in solution of iodine in iodide of 

 potassium, 



792. Gallein. — Aronson (Gentralh. vied. Wins., 1890, p. 577) stains 

 sections of material hardened in liquid of Erlicki or Miiller (these must 

 be mordanted with acetate of copper) for twelve to twenty-four hours in 

 a solution of 3 to 4 c.c. of Gallein (Grlibler & Co.) in 100 c.c. of water 

 with 20 of alcohol and three drops of concentrated solution of carbonate 

 of soda. They are then differentiated by the method of Weigert, or Pal. 

 Nei-ve-fibres red. A second stain with Methylen blue may follow (best 

 after differentiating with permanganate). Similarly Scheotteb 

 {Centralb. allg. Path., xiii, 1902, p. 512). 



793. ScHROTTEE [Neurol. Centralb., xxi, 1902, p. 338; Zeit. wiss. Mik., 

 xix, 1903, p. 381) also stains sections for two to three hours in a 5 per 

 cent, solution of sulphatizarinate of sod i, to which is added a few drops 

 of 5 per cent, oxalic acid (enough to give an orange tint), then differ- 

 entiates until no more colour comes away, in carbonate of soda solution 

 of Yifoo strength, and mounts in balsam. Myelin red, on a colourless 

 ground. 



794. Toluidin Blue and Methylen Blue. — Harris {Philadelphia 

 Med. Journ., May 14th, 1898) stains sections (of material hardened as 

 for Weigert's stain) for several hours in a 1 per cent, solution of 

 toluidin blue in 1 per cent, borax solution, and differentiates in saturated 

 aqueous solution of tannic acid. Similarly, but with methyhn blue, 

 in a complicated way Peaenkel, Neurol. Centralb., xxii, 1903, p. 7(j6 

 {Zeit. iciss., Mih., xx, 1904, p. 345). 



BiNG anil Ellbemann {Arch. Anat. Phys., Phys. Abth., 1901, p. 260) 

 harden in 9 parts of acetone to 1 of formol, cut without embedding, 

 stain for five to ten minutes in saturated Methylen blue, and put for one 

 or two into saturated picric acid. 



795. Other Modifications or Similar Methods.— Pi^ECHSia, Arch. 

 Anat. Phys., Phys. Abth., 1889, p. 537 ; Bregma, Zeit. wiss. Mil:., vii, 

 1890, p. 36; Rossi, ibid., vi, 1889, p. 182; Meeciee, ibid., vii, 1891, 

 p. 480; Haug, ibid., p. 153 ; Walsem, ibid., xi, 1894, p. 236; Bobeetson, 

 ibid., xiv, 1897, p. 80 {Brit. Med. Journ., 1897, p. 651). 



