NEUROGLIA, AND SENSE ORGANS. 441 



iron licematoxylin, and differentiating with 2 per cent, iron-alum or 

 Weigekt's borax-fen-icyanide mixture. 



See also Meves, Arch. mik. A)iat., Ixxi, 1908, p. 573. 



840. Malloet's Haematoxylin Stains {.Tourn. Ejrfer. Med., y, 

 1900, p. 19). — Tissues to be fixed, mordanted, and cut as 

 directed under Malloey, § 838. The sections are put for a 

 quarter of an hour into 0'5 per cent, solution of permanganate 

 of potash, washed and put for a quarter of an hour into 1 per 

 cent, solution of oxalic acid, well washed and stained for 

 twelve to twenty-four hours or more in Mallory's phospho- 

 tungstic ha3matoxylin. Wash, dehydrate in 95 per cent, 

 alcohol, clear with organum oil, mount in xylol-balsam. 

 Axis-cylinders and nerve-cells pink, neuroglia blue. To get 

 a more isolated stain of neuroglia, the sections should be 

 brought for five to twenty minutes, after staining, into a 30 

 per cent, alcoholic solution of dry sesquichloride of iron. 

 Neuroglia and fibrin blue, the rest colourless. 



Malloey's phospho-moZiz&cZic haematoxylin may also be 

 taken for the stain, but is less elective. 



Da Fano {Ricerche Lab. Anat. Roma, xii, 1906, p. Ill) 

 fixes in a mixture of 72 vols, of pyridinwitli 28 of nitric acid 

 of 50 per cent, and stains as Mallory. Or, he fixes in a 

 mixture of 3 vols, of nitrate of pyridin with 1 vol. of osmic acid 

 oF 1 per cent., and stains with Benda's alizarin toluidin blue. 



FiBANDT (Arch. mile. Anat., Ixxvi, 1910. p. 15) describes a very 

 compKcated modification of Mallory's pljosphotungstic haematoxylin 

 method. 



Alzheimer (quoted from Spielmeybe's " Teclcnilc d. mik. Unfersuch. 

 d. Nervensy stems," p. 106) fixes in Weigert's mordant (with formol) and 

 stains with Mallory's phoSi homolybdic hajmatoxylin. 



ElSATH (Arch. Psychiatr. u. Nervenheilh., xlviii, 1911, p. 896; Zeit. 

 wiss. Mik., 1913, p. 420) has a highly complicated modification of the 

 same stain, specially for glia granules. 



841. Anglade and Moeel {Rev. Neurol., ix, 1901, p. 157) 

 harden in a mixture of 3 parts of liquid of Fol (§ 42), with 

 1 of 7 per cent, sublimate solution, dehydrate with alcohol 

 followed by aceton, make paraffin sections and stain in 

 saturated aqueous solution of Victoria Hue, heated till it 

 steams, rinse with liquid of Gram (§ 287), differentiate with 

 xylol 1 part, anilin 2 parts, and mount in balsam. Simple, 

 applicable to lower animals, and gives very sharp images. 



