NKUROOLIA, AND SENSll ORGANS. 447 



of 0'5 per cent, to 1 per cent, solution every five minutes 

 tlu'ougli tlie vena femoralis, until the deatli of the animal. 

 'L'lie cochlea then to be got out, exposed to the air for 15 or 

 30 minutes, and fixed for some hours (overnight) in 10 per 

 cent, ammonium molybdate with a little osuiic acid. Then 

 decalcified in trichloracetic acid of 5 per cent, with a trace 

 of platinum chloride, washing for 24 hours and got into 

 paraffin . 



For fishes and amphibia the immersion method will suffice. 



850. Other Methods. — Waldeyee, Strieker's Handb., p. 968 (de- 

 calcification either in O'OOl per cent, palladium chloride containing 10 

 per cent, of HOI, or in chromic acid of 0'25 to 1 per cent.). 



Urban Peitchard [Journ. Boy. Mic. Soc, 1876, p. 211). — Decalcifi- 

 cation in 1 per cent, nitric acid. 



Latdowsky {Arch. mile. Anat., 1876, p. 497). — Fresh tissues (from the 

 cochlea) are treated -with 1 per cent, solution of silver nitrate, then 

 washed for ten minutes in water containing a few drops of 0'5 or 1 per 

 cent, osmic acid solution, and mounted in glycerin. 



Max Tlbsch {Arcli. mile. Anat., 1878, p. 300) ; Tapani {Arch. Ital. de 

 Biol., vi, p. 207) ; EiCHLBK, Abh. math-phys. CI. Silclis. Ges. Wiss., xviii, 



1892, p. 311 ; Zeit. wiss. MiJc, ix, 1893, p. 380 (injection of blood-vessels 

 of the labyrinth) ; Siebenmann, Die Blidgefdsse iin Labyr'mthe des 

 ■nieuschlicheii Ohms, Wiesbaden, Bergmann, 1894' ; Zeit. iviss. Mih., xi, 

 1894, p. 386; Geay, Joum. Anat. Phys., 1903, p. 379; Scott, ibid., xliii, 

 1909, p. 329. 



851. Oifactive Nerve-endings, Tactile Corpuscles, etc. — 

 Besides the gold method, Chapter XVII, and the metliylen- 

 blue method, Chapter XVI, the rapid bichroinate-and-silver 

 method of Golgi should be employed, and for the oifactive 

 mucosa gives the best results ; see van Gehdchten, La Cellule, 

 vi, 1890, p. 405. For intra-epidermic nerve-endings, besides 

 the methods given Chapter XXVll, the Goi.Gi method should 

 be employed. According to van Gtehuchthn {La Cellule, ix, 



1893, p. 319) it gives much better results than gold methods. 

 He uses the rapid process. For tactile corpusclen, etc., besides 

 the methods given § 661 and 662, see Ramon y Cajal's neuro- 

 fibril methods. 



