452 CHAPTER XXXVI. 



with liydroclilorio acid (in preference to nitric acid). The 

 mixture § 5^2 may also be used. If you stain with borax- 

 carmine and wash out in this mixture, the pigment will be 

 found to be removed quicker than the stain is washed out. 



Lenhossek (Zeit. iviss. Zool., Iviii, 1894, p. 636; Arch. mile. 

 All at., xlvii, 1896, p. 45) applies the method of Golgi to the 

 eyes of Cephalopods. 



Similarly KorsCH (Anat. Anz., xi, 1895, p. 362), but using 

 formol instead of the osmic acid. 



Hesse [Zeit. iciss. Zool., Ixviii, 1900, p. 418) fixes eyes of 

 Heteropoda with one of formol to 4 of water, and (p. 456) 

 bleaches those of Cephalopoda by the methods of Gtrknacheb 

 and that of Jandee, § 583. 



See also Meeton, ibid., Ixxix, 1905, p. 326. 



860. Eyes of Lamellibranchiata. — See Patten, Mittli. Zool. 

 Stat. Neapel, vi, 1886, p. 733, andEAWiTz, Jena. Zeit. Naturw., 

 xxii, 1888, p. 115, and xxiv, 1890, p. 579 (bleaches with 

 caustic soda); see § 584. Hesse {op. cit., last §, p. 380) 

 employs the method of Jandee for Area. He fixes the eye 

 of Pecten in 10 per cent, foraiol for five minutes, followed by 

 sublimate or picro-nitric acid. 



861. Shell. — Sections of non-decalcified shell are easily 

 obtained by the usual methods of grinding, or, which is often 

 a better plan, by the methods of v. Koch or Eheknbadm. 

 MosELEY {Quart. Journ. ilic. Sci. [2], xxv, 1885, p. 40) 

 decalcifies with nitric acid of 3 to 4 per cent, and then makes 

 sections. This method serves for the study of the eyes of 



CniTONIDiE. 



862. Injection of Acephala(FLEMMiNG, J.rc/7. mik. Anat., 1878, 

 p. 252). — To kill the animals freeze them in a salt-and-ice 

 mixture, and throw them for half an hour into lukewarm 

 water. They will be found dead, and the injection-pipe may 

 be tied in the heart, and the entire animal filled and covered 

 up with plaster-of-Paris, which serves to occlude cut vessels 

 that it is not possible to tie. As soon as the plaster has 

 hardened the injection may be proceeded with. See also 

 Dewitz, Anleit. zur Anfert. zootom. Pra.p., Berlin, 1886, p. 44 

 (^Anodonta) and p. 52 {Helix). 



Dakin {Liverpool Mar. Biol. Cvmvi., xvii, 1909, p. 76) 



