METHODS FOB INVBRTBBBATES. 457 



silver process. Maetin {G.R. Soc. Philomath., 1893^ p. 3) 

 injects them witli indigo white (through the body cavity) ^ 

 and puts into hot water from which the air has been expelled 

 by boiling. Trachese blue. 



872. Brain of Bees. — Kenyon (Jotirn. Corny. Neurol., vi, 

 1896, p. 137 ; Jotmi. Roy. Mic. Soc, 1897, p. 80) treats by 

 the Goi.Gi process (seldom successful), or hardens in a 

 mixture of one part formol and two of 5 per cent, sulphate 

 of copper, followed by staining in Mallory's phospho-molybdio 

 hematoxylin. 



JoNEScu {Jena. Zeif., xlv, 1909, p. Ill) has employed the 

 silver methods of Ramon y Cajal and Bielschowsky and Wolff. 



873. Ventral Cord.— Floyd {Marie. Anniv. vol. 1904, p. 

 355) fixes the ganglia of Periplaneta for eighty minutes with 

 vapour of formol, and brings into alcohol. 



See also Binet, Journ. Anat. Phys., xxx, 1894, p. 469. 



874. Eyes of Arthropods. — For the methods of Lankestee 

 and Bourne {Quart. Journ. Mic. Sci., 1883, p. 180 : Limnlus) ; 

 HiCKSON (ibid., 1885, p. 243 : Musca) ; Paekee {Bull. Mus. 

 Harvard Coll., xx, 1890, p. 1 ; Zeit. iviss. Mik., viii, 1891, 

 p. 82 : Homaru.s) see early editions. 



Paekee {Mitth. Zool. Stat. Neapel, xii, 1895, p. 1) also 

 applies the methylen blue method to the retina and optic 

 ganglia in Decapods, especially in Astacus. He injects O'l c.c. 

 of a 0'2 per cent, solution into the ventral sinus. After 

 twelve to fifteen hours the animals are killed, the ganglia 

 quickly dissected out, and the stain fixed as described, § 344. 



For his method for eyes of Scorpions see § 583. 



For the methods of Purcell for the eyes of Phalangida see 

 Zeit. wiis. Zool., Iviii, 1894, p. 1. He has the following 

 stain. The cephalothorax is removed and brought for 

 twenty minutes into 50 per cent, alcohol warmed to 46° or 

 50° C, and saturated with picric acid. The pigment 

 dissolves in this solution and stains the nuclei and some 

 other parts of the rhabdoms, so that no further stain is 

 required. 



Hennings {Zeit. wiss. Mik , xvii, IDCO, p. 326) depigments 

 sections by putting then for ten minutes (Musca) to twelve 



