458 CIIAPTEil XXXVI. 



hours (Myrioiioda) into a mixture of 2 parts of 80 per cent, 

 alcoliol with one of glycerin and 2 per cent, of nitric acidj 

 best kept at 35° C. The elements are well preserved. 



WiDMANN (Zeit. wiss. Zool., xo, 1908, p. 260) makes the 

 lens of Arachnida fit for sectioning by putting for a day or 

 so into alcohol with 10 to 15 per cent, of nitric acid ; and 

 bleaches sections with 1 part of chlorine water to 2 of alcohol. 



See also Rosenstadt, Arch. mile. Anat., xlvii, 1896, p. 478 ; 

 ViALLANES, Ann. Sci. nat., xiii, 1892, p. 354; and Dietrich, 

 Zeit. wiss. Zool., xcii, 1909, p. 465 (fixes in alcoholic formol, 

 and bleaches with dilute aqua regia). 



875. Injections (Arachnida and Crustacea especially). — 

 AiME ScHNBiDEE [Toblettes Zool., ii, 1892, p. 123) recom- 

 mends lithographic Indian ink, the animals being narcotised 

 with chloroform, then injected and thrown into strong alcohol. 

 Similary Causaed {Bull. Sc France Belg., xxix, 1896, p. 16). 



876. Aretiscoida (Doyebe, Arch. mile. Anat., 186.5, p. 10.5). — Exa- 

 mination of living animals after partial asphyxiation in boiled water. 

 See early editions. 



Vermes. 



877. Chsetopoda: Cleansing Intestine. — Kukenthal {Journ. 

 Roy. Hie. See, 1888, p. 1044) -pnts Lumh-icus intoa glass vessel 

 filled with bits of moistened blotting-paper. They gradually 

 evacuate the earthy particles from the gut, and fill it instead 

 with paper. 



YoGT and Ydng {Traite d'Anat. Camp. Prat., v) recom- 

 mend coffee-grounds instead of paper, as they cut better after 

 imbedding. 



JoEST [Arch. EiitivicMungsmech., v, 1897, p. 425) simply 

 keeps the worms for a few days in moist linen, and finds the 

 gut empty. 



Peael [Juurn. appl. Mic, iii, 1901, p. 680) injects alcohol 

 of 6 per cent, through the gut of narcotised worms. 



878. Cheetopoda : Fixation. — Lumhriciis vn.2ky he anaesthetised 

 by putting the animals into water with a feAV drops of chloro- 

 form. Peheiek puts them into water in a shallow dish, sets 

 up a watch-glass with chloroform in the corner of it, and 

 covers the whole. 



