460 OHAPTER XXXVI. 



880. Nerves of Aiinelids.— The mefchylen blae method and 

 the bichromate of silver method of Golgi [the rajnd methoi) . 

 For the latter see v. Lenhossek [Arch. mile. Anat., xxxix, 

 p. 102). 



Langdon {Journ. Gomp. Neur., x, 1900, p. 4) injects strong 

 solution of methylen blue into the body cavity of Nereis, and 

 puts the animal for some houi-s into sea-water in the dark, 

 fixes the stain by Bethe's method, and makes paraffin 

 sections. 



See also M. Lewis, Anat. Anz., xii, 1896, p. 292 ; ATHESOJf, 

 ihid., xvi, 1899, p. 497; and the methods of Apathy §§ 342, 

 368, 37], and 773. 



881. Hirudinea. — For the methods of killing see those given 

 for Lumhrlcus \n § 878, also §§ 20 to 26, and 49. 



Whitman [Meth. in ynic. Anat ^ p. 27) recommends that 

 they be killed with sublimate. 



I have obtained better results myself by narcotising with 

 carbonic acid (§ 26), and fixing with liquid of Flemraing. 

 I have also found that lemon juice kills them in a state of 

 very fair extension. 



Apathy succeeds with alcohol of 40 per cent. 



Graf {Jen. Zeit., 1893, p. 165) has obtained good results 

 by narcotising with decoction of tobacco. 



882. Injection.— Whitman [Amer. Natural, 1886, p. 318) 

 states that very perfect natural injections may often be 

 obtained from leeches that have been hardened in weak 

 chromic acid or other chromic liquid. 



Jacquet {Mitth. Zool. Stat. Neapel, 1885, p. 298), for 

 artificial injections, puts leeches into water with a very small 

 quantity of chloroform, and allows them to remain a day or 

 two in the water before injecting them. 



883. Nervous System. — Impregnation with gold. Bristol 

 {Journ. nf Morph., xv, 1898, p. 17) kills in formic acid of 15 

 to 20 per cent., puts for twenty-five minutes into 1 per cent, 

 gold chloride, reduces in formic acid of 1 per cent, (twelve 

 to eighteen hours), and imbeds in paraffin. See also §§ 342, 

 368, 371 and 773. 



884. Nephridia. — Shearer {Quart. Journ. Micr. 8ci., Iv, 

 IfllO, p. 288) stains Ilistriohdella intra vitam with very weak 



