METHODS FOE INVERTEBRATES. 483 



Van Bemengem [Journ., 1894, p. 405) fixes for a few minutes 

 witli a mixture of 1 part 2 per cent, osmic acid, and 2 parts 

 10 to 25 per cent, solution of tannin, washes, treats with 0"25 

 to 0'5 per cent, solution of nitrate of silver, then for a few 

 seconds with a mi.xture of 5 parts gallic acid, 3 of tannin, 

 10 of acetate of soda, and 350 of water, then puts back again 

 into the silver for a short time,_then washes and mounts. 



See also Stephens, ibid., 1898, p. 685, and Gordon, ibid., 

 1899, p. 235, and the methods of Teenkmann (Ceiilralb., vi, 

 1889, p. 483 ; Zeit. iciss. Mile, vii, 1890, p. 79) ; Beown 

 (Joimi. Roy. Mic. Soc, 1893, p. 268) ; Jdlien [ibid., 1894, p. 

 403) ; ScLAVO (Zeit. iuiss. Mile, xiii, 1896, p. 96) ; Hbssuet 

 (ibid., p. 96) ; MuiK {Journ. Roy. ]\lic. Soc, 1899, p. 235) ; 

 McCeoeie {ibid., 1897, p. 251; he stains for two minutes in a 

 mixture of equal parts of concentrated solution of night-blue, 

 10 per cent, solution of alum, and 10 per cent, solution of 

 tannic acid) ; Zettnow {ibid., 1899, pp. (362, 664) ; Moeton 

 {ibid., 1900, p. 131) ; Welcke {ibid., p. 132) ; Lkvaditi, C. R. 

 Soc. Biol., lix, 190-5, p. 326 (for SpirocJiWte pallida, Ramon's 

 neurofibril stain) ; Meieowsky, Miinch. Died. Woclienschr., 

 Ivii, 1010, No. 27; Kai.b, ibid.. No. 26 {Zeit. wiss. Mil:, 

 xxix, 1912, pp. 123, 124; both for Spirochete). 



