Staining 157 



Gram-negative Gram-positive 



Bacillus anthracis symptomatici; Bacillus afirogenes capsulatus; 



Bacillus coli (whole group); Bacillus anthracis; 



Bacillus ducreyi; Bacillus botulinus; 



Bacillus dysenterise; Bacillus diphtheriae; 



Bacillus icteroides; Bacillus subtilis (whole group); 



Bacillus influenzae; Bacillus tetani; 



Bacillus maUei; Bacillus tuberculosis (whole acid-fast 



Bacillus oedematis maligni; (group); 



Bacillus pestis bubonica; Diplococcus pneumonicc; 



Bacillus pneumoniie (Friedlander); Micrococcus tetragenus; 



Gram-negdtive Gram-positive 



Bacillus proteus vulgaris; Staphylococcus pyogenes albus; 



Bacillus pyocyaneus; Staphylococcus pyogenes aureus; 



Bacillus rhinosderomatis; Streptococcus pyogenes. 



Bacillus suipestifer; 

 Bacillus suisepticus; 

 Bacillus typhosus (whole group) ; 

 Diplococcus intracellularis meningitidis; 

 Micrococcus catarrhalis; 

 Micrococcus flavus; 

 Micrococcus crassus; 

 Micrococcus pharyngis siccus; 

 Micrococcus gonorrhoeae (Neisser) 

 Micrococcus melitensis; 

 Spirillum choleras asiaticse 

 Spirillum cholerae gallinarum; 

 SpirQlum cholerae nostras; 

 Spirillum metschnikovi; 

 Spirillum tyrogenum; 

 Spirochaete duttoni; 

 Spirochaete obermeieri; 

 Spirochaete refringens; 

 Spirochaete icterohemorrhagiae; 

 Spirochaete morsus muris; 

 Treponema pallidum; 

 Treponema pertenue. 



To apply the test to micro-organisms in culture or in morbid 

 fluids, the following simple method may be employed: A thin layer 

 of a suspension of the bacteria to be examined is spread upon a 

 slide or cover-glass, dried, and fixed; then flooded with the anilin-oil 

 gentian violet or other staining solution. The solution is kept warm 

 by holding the glass flooded with the stain over a small flame. The 

 process of staining is continued from two to five minutes. If the 

 heating causes the stain to evaporate, more of it must be added so 

 that it does not dry and incrust the glass. 



The stain is poured off, and replaced by Gram's solution, which 

 is allowed to remain from one-half to two minutes, and gently 

 agitated. 



The smear is next washed in 95 per cent, alcohol until the blue 

 color is wholly or almost lost, after which it can be counterstained 

 with pyronin, eosin, Bismarck brown, vesuvin, etc., washed, dried, 

 and mounted in Canada balsam. Given briefly, the method is: 



Stain with Ehrlich's solution two to five minutes; 

 Gram's solution for one-half to two minutes; 

 Wash in gs per cent, alcohol until decolorized; 



