2IJ 



The Cultivation of Anaerobic Organisms 



stituted for the cotton stopper a sterile rubber cork containing 'j 

 long entrance and short exit tube of glass, passed hydrogen througl 

 the tube until the oxygen has been entirely removed, then sealed tht 

 ends in a flame. In this tube the growth of superficial and deep 

 colonies can be observed. Hansen and Liborius constructed special 



Fig. 55 — Novy's jars for anaerobic cultures. 



Fig- S6.— Ffankel's method of 

 making anaerobic cultures. 



Fig. 57- — Liborius' tube for 

 anaerobic cultures. 



tubes by fusing a small glass tube into the wall of a culture-tube, 

 and narrowing the upper part of the tube in a flame. After inocu- 

 lation, hydrogen is passed into the small tube and permitted to es- 

 cape through the mouth of the large tube until the air is entirely 

 replaced, after which both tubes are sealed in a flame. 



Instead of having a special apparatus for each culture, it is far 

 better to adapt the principle to some larger piece of apparatus that 



