298 Wassermann Eeaction for Diagnosis of Syphilis 



quantity of the antigen that can be added without preventing hem- 

 olysis when the normal serum is used is probably 0.18 cc. At the 

 same time 0.09 cc. is the smallest quantity that can be added, when 

 the syphilitic serum is used, to prevent it. In this case the dose 

 exactly fulfils Kaplan's requirement that "The unit dose of antigen 

 must completely inhibit hemolysis : . . of a known leutic serum, 

 provided double the dose does not interfere with the complete hem- 

 olysis of cells using a known normal serum and complement." 

 We have now accomplished the titration of all five of the factors 

 involved in making the Wassermann reaction, but we have done 

 more, we have really done the test, and have seen positive and nega- 



Tubes 

 I. 



Table I. — Series with the Normal Serum 



3- 

 4- 

 S- 

 6. 



7- 

 8. 



I unit of -|- 

 complement 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 " + 



+ 



I unit of + antigen o.oi 

 normal serum 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



0.03 

 P. OS 

 0.07 

 0.08 

 0.09 



O.IO 



0.12 



°iS 

 0.18 

 0.2 



OS'S 0,-^.S 



4J 10 E q M 

 cj3 a*' a 



■*j S? o iitS 

 Si! flJ +j 



•a.S g ^ g 

 HUJ; h « 2 



Tubes 



Table II. — Series with the Syphilitic Serum 



I unit of + I unit of + antigen 0.0 1 

 complement syphilitic serum 

 " + 



4- 



6. 



+ 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 



0.03 

 0.05 



0.07 

 0.08 

 0.09 



°iS 



^ H V ^-ti 

 W^ t'- M « 



E.§||-g 

 S 0) E-5-9 



•3 b S.° 3 



.5 « P.:S I,- 

 01 3 >"■" h 



•°o g.og 



<!>...< 1) > S 



r*u-Mi 3 

 (^ o ea rt 



Complete 

 hemolysis. 



No 

 hemolysis. 



Complete 

 hemolysis. 



Sugges- 

 tion of 

 hemolysis, 

 Slight 

 hemolysis. 

 Partial 

 hemolysis. 

 No 

 hemolysis. 



