Staining 



379 



reappear in new cultures "through many generations." Noguchi's 

 paper seems more like a preliminary report than a finished work, and 

 future publication on the subject is promised. Two methods of 

 obtaining the virus of rabies freed from the cells of the host and free 

 from contaminating organisms, pubhshed by Poor and Steinhardt,* 

 give some promise of permitting the introduction of the bodies of 

 rabies into artificial culture media in a measured quantity of fluid, 

 perhaps containing a known number of organisms, and thus per- 

 mitting better methods of estimating the growth in artificial culture. 



Fig- 133. — From dog "street-virus," brain; a, b, c, and /, types of Negri 

 bodies seen at death of dog; d, e, g, and h, apparent multiplication and segmenta- 

 tion of the bodies after three days at 24°C. (Williams, in Jour. Am. Med. 

 Assoc.) 



Staining. — The Negri bodies are not difficult to stain and find 

 when one is famihar with them or when they are present in the 

 nervous tissue in considerable numbers. To find a few, to find them 

 quickly, and to recognize them unmistakably is, however, a different 

 matter. They stain by all of the Romanowsky modifications, by all 

 of the eosin-methylene blue combinations, and by various other 

 methods. 



*"Jour. of Infectious Diseases," 1913, xii, 202. 



