750 



Glanders 



conjoined individuals may occasionally be found. Long threads 

 are never formed. 



When stained with ordinary aqueous solutions of the aniline 

 dyes, or with Loffler's alkaline methylene-blue, the bacillary sub- 

 stance does not usually appear homogeneous, but, like that of the 

 diphtheria bacillus, shows marked inequalities, some areas being 

 deeply, some faintly, stained. 



The bacillus is non-motile, has no flagella, and does not form 

 spores. 



Staining. — The organism can be stained with the watery anilin- 

 dye solutions, but not by Gram's method. The bacillus readily 

 gives up the stain in the presence of decolorizing agents, so is dif- 



/■>( 



'*>-, 





fig. 293. — Bacillus mallei, from a culture upon glycerin agar-agar. X 1000 

 (Frankel and Pfeiffer). 



ficult to stain in tissues. LoflBier accomplished the staining by 

 allowing the sections to lie for some time (five minutes) in the alka- 

 line methylene-blue solution, then transferring them to a solution of 

 sulphuric and oxaHc acids: 



Concentrated sulphuric acid 2 drops 



Five per cent, oxalic acid solution i drop 



Distilled water 10 cc. 



for five seconds, then to absolute alcohol, xylol, etc. The bacilli 

 appear dark blue upon a paler ground. This method gives very good 

 results, but has been largely superseded by the use of Kiihne's car- 

 bolmethylene-blue. 



Methylene-blue .^ 1.5 



Alcohol 10. o 



Five per cent, aqueous phenol solution 100. o 



