Cultivation 



781 



bouillon, then the piece of agar-agar is to be transferred by means of 

 the platinum loop to a tube of melted sugar-agar cooled to 4o°C. 



Fig. 310. — Actinomycosis: Surface colonies oh agar and on coagulated Loffler's 

 blood serum (J. H. Wright; photograph by L. S. Brown). 



It should be immersed deeply in the agar and the tube placed in the 

 incubator. If the colony thus transferred to the agar-agar is 

 capable of growth, in the course of some days it will have formed 



Fig. 3n. — Actinomycosis: Gross appearances of sugar agar suspension cultures 

 (J. H. Wright; photograph by L. S. Brown). 



a good-sized colony from which transplants in various culture- 

 media may be made." 



