B acteriologic Diagnosis 8ii 



2. The Complement-fixation Test. — The entire culture is used as 

 an antigen, the serum of the patient and guinea-pig complement em- 

 ployed as usual. As, however, Oidium, Actinomyces, Discomyces 

 and other fungi give the same degree of fixation, the method lacks 

 precision. 



Bloch has also employed an intra-dermic injection of a sterilized 

 emulsion of the Sporotrichum for purposes of diagnosis. In twenty- 

 four hours, patients with sporotrichosis give.a marked reaction in 

 the form of an indurated nodule with a broad reddish surrounding 

 areola. 



