NUTRIENT MEDIA AND METHODS OF CULTIVATION. 



Ill 



completely removed from the gelatine. It is, however, not ea.sy to 

 be successful at first, but with practice this can Vje accomplished 

 with rapidity and pi'ecision. A preparation is then made by rubbing 



Fig. 40.— Pasteur's Laege Incubatok. 



the extremity of the needle in a droplet of water on a slide, covering 

 with a cover-glass, and examining in the fresh state, or by spreading 

 out the droplet on a cover-glass, drying, passing three times through 

 the flame, and staining with a drop of fuchsine or gentian violet. 

 Inoculations should be made in test-tubes of nutrient gelatine 



