SUPPURATION, PYEMIA, SEPTICEMIA, ERYSIPELAS. 



li. 



with Streptococcus pyogenes, ha\-e been found in cattle plague, foot 

 and mouth disease, strangles, contagious mammitis in cows, and 

 progressive tissue necrosis in mice, and thej' will be i-eferred to fully 

 in subsequent chapters. 



EXAMIXATIOX AND C'ULTIA'ATIOX OF STREPTOCOCCI, 



Cover-glass pi'eparations can be stained witli the watery solutions 

 of the aniline dyes. In some cases veiy beaiitiful preparations can 

 he obtained by using Neelseii's solution, and I'emoving excess of 



Fig. 89.— Streptococcus Pyogenes Hominis. Pure-cultures on nutrient 



gelatine. 



«, Sub-culture from agar, 

 c, Sub-culture from milk. 



b. Sub-culture from broth. 

 d. Sub-culture from milk. 



stain by rinsing in alcohol. To examine pus, milk, or broth, take 

 an ordinary platinum needle bent at the extremity into a booklet. 

 Dip it into the liquid to be examined, and spread it on a cover- 

 glass into as thin a film as possilile ; the preparation is treated 

 in the ordinary way, that is to say, the film is allowed to dry, 

 and the cover is taken up with forceps, and passed three times 

 through the flame with its prepared side uppermost. 



Gram's Method toith Eosin.^ln this way the streptococci are 

 stained blue, and stand out in marked contrast to the rest of the 

 preparation. Use freshly prepai'ed solution. Float the cover-glasses 



