272 INFECTIVE DISEASES. 



With the discharge and with scrapings from the ulcers two 

 calves were inoculated. 



Of the two calves, one was inoculated by scarification in both 

 ears ; the other, a small calf, was scarified in the left ear. Scrapings 

 from the ulcers were rubbed into the places thus prepared. In 

 addition, in the small calf an incision was made through the corium 

 in the left groin, scrapings from different ulcers on the teats were 

 well rubbed in with the blade of the scalpel, and a portion of crust 

 inserted into a small pocket in the subcutaneous tissue. In the ears 

 and the groin there were positive results. In the large brown calf 

 one of two places inoculated in the right ear passed through the 

 following changes : On the third day there was apparent vesiculation 

 and commencing formation of crust. From day to day the crust 

 thickened, and on the eighth day the crust was at its height and 

 detached at its edges. By removing the scab an Tilcer was exposed ; 

 there was slight inflammatory thickening. About the thirteenth 

 day the ulcer had quite healed. 



"Very similar appearances resulted in the ear of the smaller calf. 

 The result of inoculation in the groin was of a very much severer 

 character. In the course of two or three days the incision had 

 apparently commenced to heal by scabbing, but there was a surround- 

 ing area which was inflamed, and painful on manipulation. The 

 inflammatory thickening which resulted continued to increase around 

 the seat of inoculation, and the thickening could be felt to extend 

 deeply into the groin. Suppuration followed, and on firm pressure 

 pus welled up through the wound. The wound then showed very 

 little disposition to heal, and the calf began to exhibit marked 

 constitutional symptoms. During the second week after inoculation 

 the animal became very dull, and was reported by the attendant as 

 refusing to feed. Diarrhoea supervened, and lasted for several days, 

 and bloody urine was passed. The calf was also noticed to cough, 

 and the cough gradually increased in severit}-. Thirty-six days after 

 the date of inoculation it was decided to kill the calf and examine 

 the condition of the viscera. The appearances which were found 

 at the post-mortem examination were as follows : — 



The upper and middle lobes of each lung were adherent to the walls 

 of the chest ; there was congestion, especially of the middle lobe, and 

 patches of recent adherent lymph. Posterior parts of the upper lobes of 

 both lungs were completely consolidated, and on section varied in colour 

 from brick-red to greyish-white. The interlobular tissue was infiltrated 

 with inflammatory products, which mapped out the tissue of the lung in 

 small indurated areas, in which the tissue was granular-looking and friable. 



