INOCULATED COW-POX. 323 



of the inflammation was sometimes so great that it spread over the 

 entire arm as far as the glands of the axilla. In one case, the 

 vesicles were enormous, and the inflammation so violent, that baths, 

 poultices, fomentations, and antiphlogistic diet scarcely sufficed 

 to reduce it. The crusts when they fell off left ulcerations which 

 were very slow to undergo cicatrisation. In some cases, the vesicles 

 which resulted hollowed out the skin so' deeply that they left regular 

 holes. 



In the following year Esthn, in England, started a stock of fresh 

 vaccifle virus from the cow, and found on inoculating childi'en that 

 the new lymph was extremely active. 



In 52 the disease was regular, 



1 severe erysipelas, 

 4 erythematous eruptions of a violent character, 



2 highly inflamed ulcerated arms, 

 1 no effect after twice vaccinating, 

 8 result unknovm ; supposed to have been favourable. 



68 



Cultivated or Attenuated Lymph. — When cow-pox lymph has 

 been mitigated by successive transmission through the human subject, 

 or by cultivation on the belly of the calf, with careful selection 

 of vesicles, it will produce effects which are as follows : About 

 the end of the second day after insertion, or early on the third day, 

 a slight papular elevation is noticeable. By the fifth or sixth day, 

 it has become a distinct vesicle, of a bluish-white colour, with raised 

 margin and central cup- like depression. By the eighth day, the 

 vesicle is perfect. It is circular, pearl-coloured, distended with clear 

 lymph, and the central depression is well marked. On the same day, 

 or a httle earlier, the areola begins to appear, and gradually extends 

 to a diameter of from one to three inches, accompanied with 

 induration and tumefaction of the subjacent connective tissue. After 

 the tenth day, the areola begins to fade, and the vesicle at the same 

 time begins to dry in the centre ; the lymph becomes opaque and 

 gradually conci'etes, and by the fourteenth or fifteenth day, a hard 

 mahogany-coloured scab is formed which contracts, dries, blackens, and 

 falls off between the twentieth and twenty-fifth days. A circular, 

 depressed, foveated, and sometimes radiated scar remains behind. 

 By selecting characteristic vesicles on the calf or on the human 

 subject, and by collecting the lymph at an early stage on the fifth, 

 sixth, or seventh day, this artificial disease, commonly known as 



