ASIATIC CHOLERA. 



363 



around an enclosed bubble of air, and forming a funnel continuous 

 with the lower part of the growth ; the latter preserves for several 

 days its resemblance to a white 

 thread (Plate II., Fig. 1). In 

 about eight days, however, lique- 

 faction takes place along the 

 whole of the needle track. 



On the surface of agar- agar 

 the cviltivation develops as a 

 white, semi-transparent layer, 

 with well-defined margin. The 



appearance on blood serum is very similar ; liquefaction very slowljr 

 takes place. In broth they form a wrinkled film on the siirface, 

 there is a rapid and abundant growth at the temperature of the 



Fig. 150.— Colonies or Koch's Comma- 

 bacilli, X 6U. 



O— 



J/. .,>'' .-' -\ •«'. . ^' 



Fig. 1.51.— Covee-glass Pkepaeation 

 FKOM THE Contents of a Cholera 

 Intestine, x 600. (a) Remain.sof the 

 epithelial cells ; (h) Comma-bacillas ; 

 (c) Group of comma-baciUi (Koch). 



Fig. 152.— Covee-glass Peepakatiok 

 OP Choleea Dejecta on Damp Linen 

 (two days old), x 600. Great prolife- 

 ration of the bacilli with spirilla (a) 

 (Koch). 



blood, and the same applies to sterihsed milk ; and they will even 

 multiply in sterilised water. In potato-cultivations the microbe 

 will only grow at the temperature of the blood (37° 0.), forming a 

 slightly brown, transparent layer. Inoculation of a cultivation of 

 the bacillus in the duodenum of guinea-pigs, with and without 



