592 APPENDICES. 



are long lash-like filaments, which, by their activity set the neigh- 

 bouring blood-corpuscles in motion. Free filaments in active move- 

 ment have also been observed. Fowrth, small spherical pigmented 

 bodies about one-quarter the size of a red blood-corpuscle, which 

 exhibit amoeboid movement. 



Inoculation experiments. — Marchiafava and Celli assert that 

 inoculation of a healthy subject with blood containing the parasites 

 will produce a paroxysm of ague with development of the hsematozoa. 

 The pathogenic power of these parasites, however, has not been 

 estabhshed. There has been no cultivation of the parasite outside 

 the animal body, and reproduction of the disease with a pure culti- 

 vation. In favour of its being a pathogenic organism, Laveran 

 points out its invariable presence in some form or other in cases of 

 malaria ; the marked changes it effects in the red blood-cells ; the 

 increase in the number of the parasites in proportion to the severity 

 of the attack ; and, lastly, their disappearance after the admini- 

 stration of quinine. Others, again, have doubted the parasitic 

 nature of these bodies, and have looked upon them as representing 

 pathological changes in the blood-cells. 



Laveran first of all suggested the name Oscillaria malarise ; but 

 subsequently he recognised that these bodies belonged to the animal, 

 not to the vegetable, kingdom. Osier has suggested that, tempo- 

 rarily at any rate, the organism .should be placed in the genus 

 Hsematomonas of Mitrophanow, thus : " Genus, Hsematomonas ; 

 species, Hsematomonas malarise. Definition — Body plastic ; ovoid 

 or globose ; no differentiation of protoplasm, which contains pigment 

 grains ; flagella variable, from one to four ; highly polj'morphic, 

 occurring in (1) amoeboid form, (2) crescents, encysted form, (3) 

 sporocysts, (4) cellular free pigmented bodies." 



Examination op the H^matozoa of Laveran. 



In the Living Condition. — Select a patient by preference who has had 

 several attacks of malaria, and is markedly ansemic. Examine before the 

 invasion of the febrile paroxysm. Take two perfectly clean cover-glasses 

 and two clean slides ; wash one of the fingers of the patient with soap 

 and water, and then cleanse with alcohol ; apply a ligature, and with a 

 clean needle puncture the thin skin near the root of the nail ; touch the 

 drop of blood which collects, with a clean slide ; cover quickly with a 

 cover-glass, and gently press it if the layer of blood be too thick. 

 Examine with a -^-^ o. i. 



In Stained Preparations. — Puncture the finger again if necessary ; 

 touch the droplet of blood with a clean cover-glass ; apply another cover- 

 glass ; press them gently together, and then slide them apart ; stain with 



