APPARATUS, MATERIAL, AND REAGENTS. 



619 



'(c) Koch's solution. 



Concentrated alcoholic solution of methylene-blue 1 



Ten per cent, potash solution .... 2 



Distilled water . . . . 200 



(d) Lbffler's solution. 



Concentrated alcohoKc solution of methylene-blue 30 

 Solution of potash, 1 to 10,000 . . .100 



25. Methyl-violet. 



(a) Concentrated alcoholic solution. 

 {b) Aqueous solution. 



Methyl-violet . 



Distilled water 



(c) Koch's solution. 



Aniline water .... 

 Alcoholic solution of methyl-violet 

 Absolute alcohol 



2-25 

 100 



100 

 11 

 10 



26. Neelsen's solution. 



Dissolve fuchsine ...... 1 



In alcohol . . . . . . .10 



Add a 5 per cent, watery solution of carbolic acid 100 



27. Nitric acid, pure. 



28. Orseille (Wedl). 



Dissolve pure ammonia-free orseille in 



Absolute alcohol . . . . . .20 



Acetic acid ....... 5 



Distilled water 40 



until a dark red Uquid results. Pilter. 



29. Picric acid. 



(a) Concentrated alcoholic solution. 



(b) Saturated aqueous solution. 



30. Picro-carmine (Ranvier). 



Carmine ..... . . 1 



Distilled water . . . . . .10 



Solution of ammonia . . . . 3 



Triturate ; add cold saturated solution of picric 

 acid . . 200 



