CULTIVATION OP ANAEROBIC BACTERIA. 8i 



the corner, and a drop of pyrogallic acid solution 

 (1 : 5), at another; the cover-glass is then immediately 

 surrounded with paraflBn or Canada balsam. As soon 

 as the two drops coalesce, which, if they are large 

 enough, takes place very soon, a fluid is formed, which, 

 having a very great affinity for oxygen, absorbs it out 

 of the cavity in the slide, and thus the necessary con- 

 ditions for the development of the anaerobes are 

 provided. 



