STAINING OF FLAGELLA. 



143 



amount required to render the flagella visible is, to 

 every 16 com. of mordant add for : — 



Spirillum choleras asiaticse 

 Spirillum ruhrum . . . . 

 Spirillum Metschnikoffii . 

 Bacillus pyocyaneus ... 

 Spirillum ooncentricum 

 Bacillus mesenterious vulgat 

 Micrococcus agilis ... 

 Typhoid hacillus .... 



Bacillus suhtilis 28- 



Bacillus osdematis maligni 

 Bauschbrand bacillus . , 



The mordant can be kept for a longer time if 

 neither acid nor alkali be added to it. The addition 

 of the soda especially causes the formation in a few 

 days of copious precipitates. In spite of this, the mor- 

 dant may still be used, but quite clear preparations 

 are no longer to be obtained with it. 



