BACILLI OF CHAUBERTS DISEASE. 201 



inoculate some agar-agar, which has recently been 

 well boiled and then allowed to solidify in a slanting 

 position, and then to replace the air with hydrogen in 

 the manner described on p. 73. The tubes are then 

 carefully closed and are kept at blood heat in an 

 incubator. Then after from five to eight hours dry 

 cover-glass preparations can be prepared from them. 

 Some of these may be immediately stained with 

 aqueous aniline dyes, and thoroughly rinsed with 

 water, whilst others are put aside in order that the 

 flagella may be stained on a future occasion. These 

 bacilli may be easily stained, the preparations being 

 especially successful when f uchsine is used ; however, 

 when they are treated by Gram's method, the results 

 are unsatisfactory. 



In order to stain the flagella, thirty-five drops 

 of the caustic soda solution are added to the mordant, 

 and the experiment is conducted in the manner de- 

 scribed in Chapter XI. ; there are very numerous 

 flagella, distributed all over the body of this bacillus. 

 Occasionally, but not frequently, a peculiar formation 

 like a plait may be observed. This is often thicker 

 than the bacteria, and becomes more intensely stained. 

 It probably consists of a very great number of flagella 

 twisted up together ; this seems especially probable, 

 as such a formation very often appears to get un- 

 wound at one extremity, so that it terminates in a 

 number of small ends. As a rule these formations are 



