XXVlll DESCIRIPTION OF PLATES. 



Figs. 3 and 4. — From cover-glass preparations of the fungus teased out of the 

 new growths produced by inoculation of a calf with pus from a boy 

 suffering from pulmonary actinomycosis. Stained by Gram's method and 

 orange-rubin. The threads' are stained blue and the clubs crimson (a) 

 In the younger clubs the thread can be traced into the interior of the 

 club (J). In some of the older clubs the central portion takes a yellowish 

 stain, and in others the protoplasm is not continued as a thread, but is 

 collected into a spherical or ovoid or pear-shaped mass. In others, again, 

 irregular grains stained blue are scattered throughout the central portion 

 (Fig. 4). X 1200. 



Fig. 5. — From a pure-culture on glycerine-agar. {a) branching filaments, 

 (J) a mass of entangled filaments. Gram's method, x 1200. 



Fig. 6. — From a similar bnt older cultivation, (a) a filament with spores, 

 (J) chains of spores simulating streptococci. Gram's method, k 1200. 



DESCRIPTION OF ' PLATES XVIL AND XVIII. 

 Actinomycosis Bovis. 



Following p. 434. 



Plate XTII. 



Section of an actinomycotic tongue stained by the method of 

 Gram and with eosin. 



Fis. 1. — This illustrates the appearance which is usually seen under a low 

 power, when a section is stained by Gram's method and with eosin. The 

 central portion of a mass of thefungus is either unstained or tinged with 

 eosin, while the marginal portion is stained blue. The reverse is seen, as a 

 rule, in sections from man ; although under a low power the general appear- 

 ance of sections from these two sources is somewhat similar, x 50. 



Fig. 2. — a, i, q, d, represent the earliest recognisable forms of the ray fungus 

 in the interior of leucocytes. In e the club-forms can be recognised. In 

 /and ^ there are small stellate groups of clubs, x 500. 



Fig. 3. — A part of the section represented in Fig. 1, under a high power. The 

 marginal line of blue observed under a low power is now recognised as the 

 result of the stain being limited to the peripherally arranged clubs. At 

 (ffl) part of a rosette has undergone calcification ; the clubs are granular, 

 and have not retained the stain. At (J) and close to it there are the 

 remains of rosettes in which the process of calcification is almost complete. 

 X 500. 



Plate XVIII. 

 The figures in this plate are taken from sections of a case of 

 so-called "osteosarcoma," in which the grovirth of the fungus was 

 remarkably luxuriant. The specimens were stained by Plauts' 

 method. 



Fig. 1. — ^Diffierent forms of clubs in different specimens : x 1200. 

 (a) Very small club-shaped elements. 

 (J) A club with trapsverse segmentation. 

 (c) A club with lateral daughter clubs. 



