JUCROSCOPICAL EXAMINATIl'X OF EACTLRLi. 95 



in par-afiiEe or ct-lloidm. and mouiiTe<i c^n cork : or. if firm euongL. 

 they may be liie<l upon cork without anv embedding material at 

 all. Paradine. diss'jlve'i in cHorofoiia. will l>e found verv .■^e^Tic■e- 

 able as an eml>e<i'iing material. 



<_'ork^ ready cut for the clamp of the microtome aie <meared 

 over with the solution of celloi'iin. This can \<f- applied with a 

 gla->s r'->l t'j the surface which is to receive the piece of tis-ue- 

 The cork> are then set a.~ide for the film of celloiiin to harden. 

 In the case of lung, or degenerated broken-down tissue, the 

 ^f-ecimen should le leit for a much longer time than i~ found to 

 \v >uiiicient for fii-mer structiu-es. When ready, it is removed 

 fiijm the celloiflin s<>lutic'n with fcTcep'S and place<i upon the pre- 



F;>. J-.— Jv;-/. iiy^i.'. 



pare<l cork. Enough of the s^i.lution. which is of syrupy consistence. 

 is allowed to fall on the piece of tissue to cover it eompiletely. and 

 the mountei specimen i~ place^i in the alcohol to harden. The 

 si:>ecrmen wiJi l;>e readv for cutting next iJay. 



The specimen mav be more neatlv emh>eide<i by fixing it with 

 a piLn in a .small paper tray, pioui-ing the eelloidin .solution over it. 

 and then placing the tray in alcohol to harden the celloi'iin. Tlie 

 eml:>e«ided sjiecimen is then fixe^l on a cork, which has i:>een cut i>a- 

 the clamp of the micTotome. The cellci'lin in the section 'iisapj;>ear> 

 in the f>roc-ess of clearing with clove-oil. 



In the case of specimens embe-i'le-i in celloiiin. or motLnte<i 

 dii-ectly on a cork, the Ti->sue. as well as the blade of the knife, should 

 tie kept constantly bathe! \*ith alcohol, and the section^ transferred 

 from the blade mth a camel's-liair bru^h. and iloate>l in alcohol- 



