SUPPURATION, PYEMIA, SEPTICEMIA, ERYSIPELAS. 



It 



with 8treptoccifcns pyogenes, lia\e lieeu foimd in cattle plague, foot 

 and month disease, sti'angles, contagious mammitis in cows, and 

 progressive tissue necrosis in mice, and the_y will lie i-efei'red to fully 

 in subsequent chaptei-s. 



Examination axu C'ultiyatiox of Streptococci, 



Cover-glass pi-epai-atious can Le stained with the watery solutions 

 of the aniline dyes. In some cases vei'v beautiful jii-epa rations can 

 lie obtaineil by using Neelsen's solution, and remo\T.ni; excess of 



Yic. 89. — Streptococcus Pvoi;exes Hosiixis. Pure-cultures on nutrient 



gelatine. 



Sub-culture from agar. 

 Sub-culture from milk. 



h, Sulj-culture from Ijroth. 

 d, Sulj-culture from milk. 



stain by rinsing in alcohol. To examine pus, milk, or liroth, take 

 an ordinary platinum nee<lle bent at the extremity into a booklet. 

 Dip it into the liquid to be examined, and spread it on a cover- 

 glass into as thin a film as possiljle ; the preparation is treated 

 in the ordinary way, that is to Siiy, the film is allowed to dry, 

 and the cover is taken up with forceps, and passed three times 

 through the flame with its prepared side uppermost. 



Oram's lUethod with Eosm.^ln this way the streptococci are 

 stained blue, and stand out in marked contrast to the rest of the 

 preparation. Use freshly prepared solution. Float the cover-glasses 



