190 INFECTIVE DISEASES. 



The cocci are found in pus from the urethra and other mucous 

 membranes affected by the disease. They have also been found in 

 urethral and inguinal abscesses in association with Staphylococcus 

 pyogenes aureus. 



Method of Staining. 



Cover-glass preparations are made in the usual way, and 

 double stained with Loffler's methylene blue, and eosin. 



Schutz recommends floating the cover-glasses for five or ten 

 minutes in a saturated solution of methylene blue in 5 per cent, 

 solution of- carbolic acid. They are washed in water, rinsed in very 

 weak acetic acid, and again washed in water. Safranin mdy be 

 used as a contrast stain. 



Fig. 91.— Gonocooous x 800 (Bumm). 

 a, free cocci ; 6, cocci in pus cells ; c, epithelial cell containing cocci. 



Egyptian Ophthalmia. 



There are two forms of ophthalmia in Egypt, one associated 

 with Gonococcus and the other with a bacillus closely resembling 

 the bacillus of mouse-septicsemia, but there are minute differences. 



Bacillus of Ophthalmia (Koch and Kartulis). Minute rods 

 which do not grow on gelatine but readily on blood serum and 

 nutrient agar, forming a plainly visible, whitish-grey shining growth. 

 Animals are insusceptible, but cultures produced the disease in the 

 human conjunctiva in two out of six cases. 



