J18 



INFECTIVE DISEASES. 



Involution forms are freely developed 

 cultures made in unsuitable media. The 



in old cultures, and in 

 bacilli possess numerous 

 flagella, and their 

 power of movement at 

 once distinguishes them 

 from anthrax bacilli. 

 They can be cultivated 

 in the ordinary media 

 in the absence of oxy- 

 gen, but more readily 

 with the addition of 

 grape- sugar or glyce- 

 rine. Radiating fila- 

 ments grow out from 

 the more or less spher- 

 ical colonies directly 



Tig. 101. Bacilli of Quakter-evil x 1000. From 

 an agar culture (Fkankbl and Pi'EIfi'BR). 



liquefaction commences. In the depth of 

 nutrient gelatine the growth occurs in two 

 or three days at 20° to 25° 0. towards the 

 lower part of the track of the inoculating 

 needle. The gelatine slowly ILquefi^es, and 

 there is considerable formation of gas with 

 the development of a peculiar odour. Spore- 

 formation occurs freely in cultures, but not 

 in the blood of infected animals until after 

 death. 



Gruinea-pigs inoculated with a pure- 

 culture, or with spore-bearing threads, die 

 in twenty-four to thirty-six hours. An em- 

 physematous infiltration with sanguineous 

 serum is produced at the seat of inoculation, 

 and the surrounding muscles are of a dark ' 

 colour. The internal organs are more or less j-j^ iu2. Pure-Culture op 

 congested. The bacilli are found in the Bacilli op Quarter-evil 



local exudation and in the surrounding '" Grape-sugar Gbla- 



. TINE (Frankbl and 



tissue, and some hours after death m Ppeipper) 



