ASIATIC CHOLERA. 



363 



around an enclosed bubble of air, and forming a funnel continuous 



with the lower part of the growth ; the latter preserves for several 



days its resemblance to a white 



thread (Plate II., Fig. 1). In 



about eight days, however, hque- 



faction takes place along the 



whole of the needle track. 



On the surface of agar-agar 

 the cultivation develops as a 

 white, semi-transparent layer, 

 with well-defined margin. The 



appearance on blood serum is very similar ; liquefaction very slowly 

 takes place. In broth they form a wrinkled film on the surface, 

 there is a rapid and abundant growth at the temperature of the 



Fig. 150. — Colonibs ov Koch's Comma- 

 bacilli, X 60. 





Fig. 15L — Oovbk-glass Peepakation 

 PBOM THE Contents of a Choleka 

 Intestine, x 600. (a) Remains of the 

 epithelial cells ; (6) Comma-bacillus ; 

 (c) Group of comma-baciUi (Koch). 



Fig. 152.— Cover-glass Peeparation 

 op Choleea Dejecta ok Damp Linen 

 (two days old), x 600. Great prolife- 

 ration of the bacilli with spirUla (a) 

 (Koch). 



blood, and the same applies to sterilised milk ; and they will even 

 multiply in sterilised water. In potato- cultivations the microbe 

 will only grow at the temperature of the blood (37° C), forming a 

 slightly brown, transparent layer. Inoculation of a cultivation of 

 the baciUus in the duodenum of guinea-pigs, with and without 



